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[Conditions of feasibility and stability in the measurement of protein synthesis using 15N glycine in intensive care patients].

Authors
  • François, G
  • Rosé, F
  • Reynier, J P
  • Dumont, J C
Type
Published Article
Journal
Annales Françaises d Anesthésie et de Réanimation
Publisher
Elsevier
Publication Date
Jan 01, 1987
Volume
6
Issue
3
Pages
178–181
Identifiers
PMID: 3619153
Source
Medline
License
Unknown

Abstract

The rates of nitrogen flux and protein synthesis in the whole body were measured in two intensive care (ICU) patients on two occasions separated by a period of 24 h. Rates of flux and synthesis were estimated from 15N excretion in urinary ammonia after a slow injection of 15N glycine over a period of 1 h. The aim of the present study was to evaluate the feasibility and the reproducibility of the method. The variation of nitrogen flux was between 6 and 9%, and for synthesis between 7 and 9%. Skeletal muscle protein breakdown was measured from urinary 3, methylhistidine excretion. The muscle contribution to the whole body breakdown rate was 32 and 41% for one patient and 48 and 51% for the other one. The combination of measurement of 15N excretion after a single dose of 15N glycine with that of urinary 3, methylhistidine provided a reproducible method for measuring whole body protein synthesis in ICU patients. It could be repeated at short intervals and gave useful comparative information provided that conditions were carefully standardized.

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