Affordable Access

Access to the full text

Conditional islet hypovascularisation does not preclude beta cell expansion during pregnancy in mice

Authors
  • Staels, Willem1, 2
  • Heremans, Yves1
  • Leuckx, Gunter1
  • Van Gassen, Naomi1
  • Salinno, Ciro1
  • De Groef, Sofie1
  • Cools, Martine2
  • Keshet, Eli3
  • Dor, Yuval3
  • Heimberg, Harry1
  • De Leu, Nico1, 4, 5
  • 1 Vrije Universiteit Brussel, Beta Cell Neogenesis (BENE), Laarbeeklaan 103, Brussels, 1090, Belgium , Brussels (Belgium)
  • 2 Ghent University Hospital and Ghent University, Department of Paediatrics, Division of Paediatric Endocrinology, Ghent, Belgium , Ghent (Belgium)
  • 3 Hebrew University Hadassah Medical School, Department of Developmental Biology and Cancer Research, Institute of Medical Research Israel-Canada, Jerusalem, Israel , Jerusalem (Israel)
  • 4 Universitair Ziekenhuis Brussel, Department of Endocrinology, Brussels, Belgium , Brussels (Belgium)
  • 5 Algemeen Stedelijk Ziekenhuis Aalst, Department of Endocrinology, Aalst, Belgium , Aalst (Belgium)
Type
Published Article
Journal
Diabetologia
Publisher
Springer-Verlag
Publication Date
Mar 16, 2017
Volume
60
Issue
6
Pages
1051–1056
Identifiers
DOI: 10.1007/s00125-017-4243-1
Source
Springer Nature
Keywords
License
Yellow

Abstract

Aims/hypothesisEndothelial–endocrine cell interactions and vascular endothelial growth factor (VEGF)-A signalling are deemed essential for maternal islet vascularisation, glucose control and beta cell expansion during mouse pregnancy. The aim of this study was to assess whether pregnancy-associated beta cell expansion was affected under conditions of islet hypovascularisation.MethodsSoluble fms-like tyrosine kinase 1 (sFLT1), a VEGF-A decoy receptor, was conditionally overexpressed in maternal mouse beta cells from 1.5 to 14.5 days post coitum. Islet vascularisation, glycaemic control, beta cell proliferation, individual beta cell size and total beta cell volume were assessed in both pregnant mice and non-pregnant littermates.ResultsConditional overexpression of sFLT1 in beta cells resulted in islet hypovascularisation and glucose intolerance in both pregnant and non-pregnant mice. In contrast to non-pregnant littermates, glucose intolerance in pregnant mice was transient. sFLT1 overexpression did not affect pregnancy-associated changes in beta cell proliferation, individual beta cell size or total beta cell volume.Conclusions/interpretationReduced intra-islet VEGF-A signalling results in maternal islet hypovascularisation and impaired glycaemic control but does not preclude beta cell expansion during mouse pregnancy.

Report this publication

Statistics

Seen <100 times