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Concurrent Longitudinal EPR Monitoring of Tissue Oxygenation, Acidosis, and Reducing Capacity in Mouse Xenograft Tumor Models

Authors
  • Bobko, Andrey A.1, 2
  • Evans, Jason3
  • Denko, Nicholas C.3
  • Khramtsov, Valery V.1, 2
  • 1 West Virginia University, In Vivo Multifunctional Magnetic Resonance Center, Robert C. Byrd Health Science Center, Morgantown, WV, 26506, USA , Morgantown (United States)
  • 2 West Virginia University School of Medicine, Department of Biochemistry, Morgantown, WV, 26506, USA , Morgantown (United States)
  • 3 The Ohio State University, The James Comprehensive Cancer Center, Columbus, OH, USA , Columbus (United States)
Type
Published Article
Journal
Cell Biochemistry and Biophysics
Publisher
Springer-Verlag
Publication Date
May 18, 2016
Volume
75
Issue
2
Pages
247–253
Identifiers
DOI: 10.1007/s12013-016-0733-x
Source
Springer Nature
Keywords
License
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Abstract

Tissue oxygenation, extracellular acidity, and tissue reducing capacity are among crucial parameters of tumor microenvironment (TME) of significant importance for tumor pathophysiology. In this paper, we demonstrate the complementary application of particulate lithium octa-n-butoxy-naphthalocyanine and soluble nitroxide paramagnetic probes for monitoring of these TME parameters using electron paramagnetic resonance (EPR) technique. Two different types of therapeutic interventions were studied: hypothermia and systemic administration of metabolically active drug. In summary, the results demonstrate the utility of EPR technique for non-invasive concurrent longitudinal monitoring of physiologically relevant chemical parameters of TME in mouse xenograft tumor models, including that under therapeutic intervention.

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