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Concurrent Longitudinal EPR Monitoring of Tissue Oxygenation, Acidosis, and Reducing Capacity in Mouse Xenograft Tumor Models.

Authors
  • Bobko, Andrey A1, 2
  • Evans, Jason3
  • Denko, Nicholas C3
  • Khramtsov, Valery V4, 5
  • 1 In Vivo Multifunctional Magnetic Resonance Center, Robert C. Byrd Health Science Center, West Virginia University, Morgantown, WV, 26506, USA.
  • 2 Department of Biochemistry, West Virginia University School of Medicine, Morgantown, WV, 26506, USA.
  • 3 The James Comprehensive Cancer Center, The Ohio State University, Columbus, OH, USA.
  • 4 In Vivo Multifunctional Magnetic Resonance Center, Robert C. Byrd Health Science Center, West Virginia University, Morgantown, WV, 26506, USA. [email protected]
  • 5 Department of Biochemistry, West Virginia University School of Medicine, Morgantown, WV, 26506, USA. [email protected]
Type
Published Article
Journal
Cell Biochemistry and Biophysics
Publisher
Springer-Verlag
Publication Date
June 2017
Volume
75
Issue
2
Pages
247–253
Identifiers
DOI: 10.1007/s12013-016-0733-x
PMID: 27193607
Source
Medline
Keywords
License
Unknown

Abstract

Tissue oxygenation, extracellular acidity, and tissue reducing capacity are among crucial parameters of tumor microenvironment (TME) of significant importance for tumor pathophysiology. In this paper, we demonstrate the complementary application of particulate lithium octa-n-butoxy-naphthalocyanine and soluble nitroxide paramagnetic probes for monitoring of these TME parameters using electron paramagnetic resonance (EPR) technique. Two different types of therapeutic interventions were studied: hypothermia and systemic administration of metabolically active drug. In summary, the results demonstrate the utility of EPR technique for non-invasive concurrent longitudinal monitoring of physiologically relevant chemical parameters of TME in mouse xenograft tumor models, including that under therapeutic intervention.

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