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Concordance analysis between estimation methods of milk fatty acid content

Authors
  • Rodriguez, Mary Ana Petersen
  • Petrini, Juliana
  • Ferreira, Evandro Maia
  • Mourão, Luciana Regina Mangeti Barreto
  • Salvian, Mayara
  • Cassoli, Laerte Dagher
  • Pires, Alexandre Vaz
  • Machado, Paulo Fernando
  • Mourão, Gerson Barreto1
  • 1 University of São Paulo
Type
Published Article
Journal
Food Chemistry
Publisher
Elsevier
Publication Date
Jan 01, 2014
Accepted Date
Jan 26, 2014
Volume
156
Pages
170–175
Identifiers
DOI: 10.1016/j.foodchem.2014.01.092
Source
Elsevier
Keywords
License
Unknown

Abstract

Considering the milk fatty acid influence on human health, the aim of this study was to compare gas chromatography (GC) and Fourier transform infrared (FTIR) spectroscopy for the determination of these compounds. Fatty acid content (g/100g of fat) were obtained by both methods and compared through Pearson’s correlation, linear Bayesian regression, and the Bland–Altman method. Despite the high correlations between the measurements (r=0.60–0.92), the regression coefficient values indicated higher measures for palmitic acid, oleic acid, unsaturated and monounsaturated fatty acids and lower values for stearic acid, saturated and polyunsaturated fatty acids estimated by GC in comparison to FTIR results. This inequality was confirmed in the Bland–Altman test, with an average bias varying from −8.65 to 6.91g/100g of fat. However, the inclusion of 94% of the samples into the concordance limits suggested that the variability of the differences between the methods was constant throughout the range of measurement. Therefore, despite the inequality between the estimates, the methods displayed the same pattern of milk fat composition, allowing similar conclusions about the milk samples under evaluation.

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