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Concentrated extract of Prunus mume fruit exerts dual effects in 3T3-L1 adipocytes by inhibiting adipogenesis and inducing beiging/browning

Authors
  • Bu, Su
  • Yuan, Chunying
  • Cao, Fuliang
  • Xu, Qifeng
  • Zhang, Yichun
  • Ju, Ronghua
  • Chen, Longyun
  • Li, Zhong
Type
Published Article
Journal
Food & Nutrition Research
Publisher
Open Academia
Publication Date
Oct 29, 2021
Volume
65
Identifiers
DOI: 10.29219/fnr.v65.5492
PMID: 34776833
PMCID: PMC8559450
Source
PubMed Central
Keywords
Disciplines
  • Original Article
License
Unknown

Abstract

Background The fruit Prunus mume has beneficial effects in the treatment of obesity and metabolic syndrome. However, its mechanism of action is unclear. Objective We assessed the effect of a concentrated water extract of P. mume fruit (CEPM) on adipogenesis and beiging/browning in 3T3-L1 cells. Methods The cell viability was determined by MTT assay. Lipid accumulation was assessed with Oil Red O (ORO) staining under different concentrations of CEPM. The effects of CEPM treatment during differentiation on beiging/browning and mitochondrial biogenesis in 3T3-L1 cells were investigated. Results CEPM treatment suppressed differentiation and decreased lipid accumulation by downregulating the expression of key adipogenic genes, including PPARγ, C/EBPα, SREBP-1c, FAS, and perilipin A. In contrast, CEPM treatment increased the mitochondrial DNA (mtDNA) content and mRNA levels of mitochondrial biogenesis genes, including NAMPT , Nrf1 , Nrf2, and CPT1 α, and reduced reactive oxygen species levels. Importantly, CEPM increased the expression of brown/beige hallmark genes ( Pgc-1 α, Ucp1 , Cidea , Cox7α1 , Cox8b , Cd137 , and Pdk-4 ), as well as proteins (UCP1, PGC-1α, NRF1, TBX1, and CPT1α). The high-performance liquid chromatography (HPLC) analysis reveals that CEPM contains mumefural, naringin, 5-HMF, citric acid, caffeic acid, and hesperidin. Conclusion The first evidence we provided showed that CEPM has a dual role in 3T3-L1 cells inhibiting adipogenesis and promoting beiging/browning, and hence, could be a potential agent in the fight against obesity.

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