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Computerized Evaluation of the Immunoexpression of Ki-67 Protein in Odontogenic Keratocyst and Dentigerous Cyst.

Authors
  • Portes, Juliana1, 2
  • Cunha, Karin Soares Gonçalves3, 4
  • da Silva, Licínio Esmeraldo5, 4
  • da Silva, Anna Karoline Fausto6, 4
  • Conde, Danielle Castex3, 4
  • Silva Junior, Arley3, 4
  • 1 Graduate Program in Pathology, School of Medicine, Universidade Federal Fluminense, Niterói, Rio de Janeiro, Brazil. [email protected] , (Brazil)
  • 2 Hospital Universitário Antônio Pedro - Rua Marquês do Paraná, 303/4th Floor, Room 1, Niterói, RJ, 24033-900, Brazil. [email protected] , (Brazil)
  • 3 Graduate Program in Pathology, School of Medicine, Universidade Federal Fluminense, Niterói, Rio de Janeiro, Brazil. , (Brazil)
  • 4 Hospital Universitário Antônio Pedro - Rua Marquês do Paraná, 303/4th Floor, Room 1, Niterói, RJ, 24033-900, Brazil. , (Brazil)
  • 5 Department of Statistics, Institute of Statistics and Mathematics, Universidade Federal Fluminense, Niterói, Rio de Janeiro, Brazil. , (Brazil)
  • 6 Immunohistochemistry Technique, Universidade Federal Fluminense, Niterói, Rio de Janeiro, Brazil. , (Brazil)
Type
Published Article
Journal
Head and neck pathology
Publication Date
Sep 01, 2020
Volume
14
Issue
3
Pages
598–605
Identifiers
DOI: 10.1007/s12105-019-01077-3
PMID: 31552621
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Evaluation and comparison of odontogenic keratocysts and detigerous cysts immunoexpression and immunostaining intensities of Ki-67 antigen by assessing the whole extent of the epithelium (all epithelium layers in combination) and each layer individually. Ki-67 immunoexpression was evaluated in 15 odontogenic keratocysts and 6 dentigerous cysts using automated methods and the Aperio Technologies Inc. computer system. No statistically significant differences were observed in immunoexpression nor in immunostaining intensities between both lesions. Also, no statistically significant differences were found between odontogenic keratocysts from maxilla versus mandible nor primary versus recurrent. However, odontogenic keratocyst showed a significantly higher cellular proliferation index in the suprabasal layers compared to the basal layer. Assessment of the cellular proliferation index through a computerized system enabled the evaluation of all epithelial tissue without field selection. The increased Ki-67 immunoexpression in suprabasal layers of odontogenic keratocyst suggests a different biological behavior and more aggressive proliferation potential when compared to dentigerous cyst. The same result was found in recurrent odontogenic keratocysts when compared with primary ones. The odontogenic keratocysts of the maxilla and mandible have similar Ki-67 immunoexpression. The evaluation of cellular proliferation only by immunohistochemical analysis with Ki-67 antigen does not provide enough data to elucidate the biological behavior of odontogenic keratocyst.

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