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Comprehensive transcriptome and improved genome annotation of Bacillus licheniformis WX-02.

Authors
  • Guo, Jing
  • Cheng, Gang
  • Gou, Xiang-Yong
  • Xing, Feng
  • Li, Sen
  • Han, Yi-Chao
  • Wang, Long
  • Song, Jia-Ming
  • Shu, Cheng-Cheng
  • Chen, Shou-Wen
  • Chen, Ling-Ling
Type
Published Article
Journal
FEBS Letters
Publisher
Wiley (John Wiley & Sons)
Publication Date
Aug 18, 2015
Volume
589
Issue
18
Pages
2372–2381
Identifiers
DOI: 10.1016/j.febslet.2015.07.029
PMID: 26226425
Source
Medline
Keywords
License
Unknown

Abstract

The updated genome of Bacillus licheniformis WX-02 comprises a circular chromosome of 4286821 base-pairs containing 4512 protein-coding genes. We applied strand-specific RNA-sequencing to explore the transcriptome profiles of B. licheniformis WX-02 under normal and high-salt conditions (NaCl 6%). We identified 2381 co-expressed gene pairs constituting 871 operon structures. In addition, 1169 antisense transcripts and 90 small RNAs were detected. Systematic comparison of differentially expressed genes under different conditions revealed that genes involved in multiple functions were significantly repressed in long-term high salt adaptation process. Genes related to promotion of glutamic acid synthesis were activated by 6% NaCl, potentially explaining the high yield of γ-PGA under salt condition. This study will be useful for the optimization of crucial metabolic activities in this bacterium.

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