Affordable Access

deepdyve-link
Publisher Website

A comprehensive profiling of sulfatides in myelin from mouse brain using liquid chromatography coupled to high-resolution accurate tandem mass spectrometry.

Authors
  • Pintado-Sierra, M1
  • García-Álvarez, I2
  • Bribián, A3
  • Medina-Rodríguez, E M4
  • Lebrón-Aguilar, R5
  • Garrido, L6
  • de Castro, F3
  • Fernández-Mayoralas, A1
  • Quintanilla-López, J E7
  • 1 Instituto de Química Orgánica General (CSIC), Juan de la Cierva 3, 28006, Madrid, Spain. , (Spain)
  • 2 Instituto de Química Orgánica General (CSIC), Juan de la Cierva 3, 28006, Madrid, Spain; Grupo de Neurobiología del Desarrollo-GNDe, Hospital Nacional de Parapléjicos, Finca "La Peraleda", 45071, Toledo, Spain. , (Spain)
  • 3 Grupo de Neurobiología del Desarrollo-GNDe, Hospital Nacional de Parapléjicos, Finca "La Peraleda", 45071, Toledo, Spain; Grupo de Neurobiología del Desarrollo-GNDe, Instituto Cajal (CSIC), Doctor Arce 37, 28002, Madrid, Spain. , (Spain)
  • 4 Grupo de Neurobiología del Desarrollo-GNDe, Hospital Nacional de Parapléjicos, Finca "La Peraleda", 45071, Toledo, Spain. , (Spain)
  • 5 Instituto de Química-Física 'Rocasolano' (CSIC), Serrano 119, 28006, Madrid, Spain. , (Spain)
  • 6 Instituto de Ciencia y Tecnología de Polímeros (CSIC), Juan de la Cierva 3, 28006, Madrid, Spain. , (Spain)
  • 7 Instituto de Química-Física 'Rocasolano' (CSIC), Serrano 119, 28006, Madrid, Spain. Electronic address: [email protected] , (Spain)
Type
Published Article
Journal
Analytica chimica acta
Publication Date
Jan 25, 2017
Volume
951
Pages
89–98
Identifiers
DOI: 10.1016/j.aca.2016.11.054
PMID: 27998489
Source
Medline
Keywords
License
Unknown

Abstract

Sulfatides are sulfoglycolipids found in the myelin sheath. The composition ratio of sulfatide molecular species changes with age, and it has also been associated with the pathogenesis of various human central nervous system diseases. However, profiling sulfatides in biological samples is difficult, due to the great variety of molecular species. In this work, a new, easy and reliable liquid chromatography-electrospray tandem mass spectrometry (LC-ESI(+)-MS/MS) method has been developed to profile sulfatide content in biological samples of myelin. The 'wrong-way-round' ionization effect has been described for this type of molecules for the first time, making it possible to correctly identify as many as 37 different sulfatides in mouse brain myelin samples, including molecules with different fatty acid chain lengths and varying degrees of unsaturation and hydroxylation. A chemometric analysis of their relative abundances showed that the main difference among individuals of different ages was the content of sulfatides with odd-numbered fatty acid chains, in addition to hydroxylated species.

Report this publication

Statistics

Seen <100 times