Comprehensive Analysis of Serum and Fecal Bile Acid Profiles and Interaction with Gut Microbiota in Primary Biliary Cholangitis.

Weihua Chen; Yiran Wei; Aizhen Xiong; Yanmei Li; Huida Guan; Qixia Wang; Qi Miao; Zhaolian Bian; Xiao Xiao; Min Lian; Jun Zhang; Bo Li; Qin Cao; Zhuping Fan; Weici Zhang; Dekai Qiu; Jingyuan Fang; M Eric Gershwin; Li Yang; Ruqi Tang; Xiong Ma

doi:10.1007/s12016-019-08731-2

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Comprehensive Analysis of Serum and Fecal Bile Acid Profiles and Interaction with Gut Microbiota in Primary Biliary Cholangitis.

Authors

Chen, Weihua¹
Wei, Yiran¹
Xiong, Aizhen²
Li, Yanmei¹
Guan, Huida²
Wang, Qixia¹
Miao, Qi¹
Bian, Zhaolian³
Xiao, Xiao¹
Lian, Min¹
Zhang, Jun¹
Li, Bo¹
Cao, Qin⁴
Fan, Zhuping⁴
Zhang, Weici⁵
Qiu, Dekai¹
Fang, Jingyuan¹
Gershwin, M Eric⁵
Yang, Li⁶
Tang, Ruqi⁷
Ma, Xiong⁸

And 1 more

¹ Division of Gastroenterology and Hepatology, Key Laboratory of Gastroenterology and Hepatology, Ministry of Health, State Key Laboratory for Oncogenes and Related Genes, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai Institute of Digestive Disease, 145 Middle Shandong Road, Shanghai, 200001, China. , (China)
² The MOE Key Laboratory of Standardization of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, China. , (China)
³ Nantong Institute of Liver Disease, Department of Gastroenterology and Hepatology, Nantong Third People's Hospital, Nantong University, 60 Middle Qingnian Road, Nantong, Jiangsu, China. , (China)
⁴ Department of Health Manage Center, School of Medicine, RenJi Hospital, Shanghai Jiao Tong University, Shanghai, China. , (China)
⁵ Division of Rheumatology, Department of Medicine, Allergy and Clinical Immunology, University of California at Davis, Davis, CA, USA.
⁶ The MOE Key Laboratory of Standardization of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, China. [email protected] , (China)
⁷ Division of Gastroenterology and Hepatology, Key Laboratory of Gastroenterology and Hepatology, Ministry of Health, State Key Laboratory for Oncogenes and Related Genes, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai Institute of Digestive Disease, 145 Middle Shandong Road, Shanghai, 200001, China. [email protected] , (China)
⁸ Division of Gastroenterology and Hepatology, Key Laboratory of Gastroenterology and Hepatology, Ministry of Health, State Key Laboratory for Oncogenes and Related Genes, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai Institute of Digestive Disease, 145 Middle Shandong Road, Shanghai, 200001, China. [email protected] , (China)

Type

Published Article

Journal

Clinical Reviews in Allergy & Immunology

Publisher

Springer-Verlag

Publication Date

Feb 01, 2020

Volume

58

Issue

1

Pages

25–38

Identifiers

DOI: 10.1007/s12016-019-08731-2

PMID: 30900136

Source

Medline

Keywords

Language

English

License

Unknown

Abstract

Accumulation of bile acids (BAs) contributes significantly to the pathogenesis of primary biliary cholangitis (PBC). Here, we sought to systematically characterize the serum and fecal BA profiles and the linkage between BAs and gut microbiota in PBC. The serum and fecal BAs were compared between 65 UDCA treatment-naive PBC and 109 healthy controls using UPLC-MS in cross-sectional study. In a prospective study, a subgroup of patients was enrolled for BA and microbiota analysis before and after UDCA therapy. BA compositions in serum and feces significantly differed between treatment-naive PBC and controls. Particularly, PBC was associated with decreased conversions of conjugated to unconjugated, and primary to secondary BAs, indicating impaired microbial metabolism of BAs. PBC patients at advanced stage exhibited a more abnormal BA profile compared with early-stage patients. UDCA treatment led to a decreased level of taurine-conjugated BAs, thereby reversing the conjugated/unconjugated ratio in PBC. Moreover, the level of secondary BAs such as DCA and conjugated DCA inversely correlated with PBC-enriched gut microbes (e.g., Veillonella, Klebsiella), while positively correlated with control-enriched microbes (e.g., Faecalibacterium, Oscillospira). Microbiota analysis also revealed a significant increase of taurine-metabolizing bacteria Bilophila spp. in patients after UDCA, which was strongly correlated with decreased taurine-conjugated BAs. In addition, serum FGF19 was remarkably increased in treatment-naïve PBC and decreased after UDCA. Our study established specific alterations of BA compositions in serum and feces of PBC, suggesting the potential for using BAs for diagnosis, and highlighting the possibility of modulating BA profile by altering gut microbiota. Graphical Abstract.

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. This record was last updated on 01/04/2021 and may not reflect the most current and accurate biomedical/scientific data available from NLM. The corresponding record at NLM can be accessed at https://www.ncbi.nlm.nih.gov/pubmed/30900136

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