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Completion of neuronal migration regulated by loss of Ca2+ transients

Authors
  • Tatsuro Kumada
  • Hitoshi Komuro
Publisher
National Academy of Sciences
Publication Date
May 18, 2004
Source
PMC
Keywords
License
Unknown

Abstract

The migration of immature neurons constitutes one of the major processes by which the central nervous system takes shape. Completing the migration at the final destination requires the loss of cell body motility, but little is known about the signaling mechanisms underlying this process. Here, we show that a loss of transient Ca2+ elevations triggers the completion of cerebellar granule cell migration. Simultaneous observation of the intracellular Ca2+ levels and cell movement in cerebellar slices of the early postnatal mice revealed that granule cells exhibit distinct frequencies of the transient Ca2+ elevations as they migrate in different cortical layers, and complete the migration only after the loss of Ca2+ elevations. The reduction of the Ca2+ elevation frequency by decreasing Ca2+ influx, or by inhibiting the activity of phospholipase C, PKC, or Ca2+/calmodulin, halted the granule cell movement prematurely. In contrast, increasing the Ca2+ elevation frequency by increasing Ca2+ release from internal stores, or by elevating intracellular cAMP levels, significantly delayed the completion of granule cell migration. The timing of the loss of Ca2+ elevations was intrinsically set in the granule cells and influenced by external cues. These results suggest that Ca2+ signaling, dictated by multiple signaling systems, functions as a mediator for completing the migration of immature neurons.

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