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Complete replication of hepatitis C virus in cell culture.

Authors
  • Lindenbach, Brett D
  • Evans, Matthew J
  • Syder, Andrew J
  • Wölk, Benno
  • Tellinghuisen, Timothy L
  • Liu, Christopher C
  • Maruyama, Toshiaki
  • Hynes, Richard O
  • Burton, Dennis R
  • McKeating, Jane A
  • Rice, Charles M
Type
Published Article
Journal
Science
Publisher
American Association for the Advancement of Science (AAAS)
Publication Date
Jul 22, 2005
Volume
309
Issue
5734
Pages
623–626
Identifiers
PMID: 15947137
Source
Medline
License
Unknown

Abstract

Many aspects of the hepatitis C virus (HCV) life cycle have not been reproduced in cell culture, which has slowed research progress on this important human pathogen. Here, we describe a full-length HCV genome that replicates and produces virus particles that are infectious in cell culture (HCVcc). Replication of HCVcc was robust, producing nearly 10(5) infectious units per milliliter within 48 hours. Virus particles were filterable and neutralized with a monoclonal antibody against the viral glycoprotein E2. Viral entry was dependent on cellular expression of a putative HCV receptor, CD81. HCVcc replication was inhibited by interferon-alpha and by several HCV-specific antiviral compounds, suggesting that this in vitro system will aid in the search for improved antivirals.

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