Affordable Access

deepdyve-link
Publisher Website

Complete genome sequence of a novel Plum pox virus strain W isolate determined by 454 pyrosequencing.

Authors
  • Sheveleva, Anna
  • Kudryavtseva, Anna
  • Speranskaya, Anna
  • Belenikin, Maxim
  • Melnikova, Natalia
  • Chirkov, Sergei
Type
Published Article
Journal
Virus Genes
Publisher
Springer-Verlag
Publication Date
Oct 01, 2013
Volume
47
Issue
2
Pages
385–388
Identifiers
DOI: 10.1007/s11262-013-0946-7
PMID: 23813250
Source
Medline
License
Unknown

Abstract

The near-complete (99.7 %) genome sequence of a novel Russian Plum pox virus (PPV) isolate Pk, belonging to the strain Winona (W), has been determined by 454 pyrosequencing with the exception of the thirty-one 5'-terminal nucleotides. This region was amplified using 5'RACE kit and sequenced by the Sanger method. Genomic RNA released from immunocaptured PPV particles was employed for generation of cDNA library using TransPlex Whole transcriptome amplification kit (WTA2, Sigma-Aldrich). The entire Pk genome has identity level of 92.8-94.5 % when compared to the complete nucleotide sequences of other PPV-W isolates (W3174, LV-141pl, LV-145bt, and UKR 44189), confirming a high degree of variability within the PPV-W strain. The isolates Pk and LV-141pl are most closely related. The Pk has been found in a wild plum (Prunus domestica) in a new region of Russia indicating widespread dissemination of the PPV-W strain in the European part of the former USSR.

Report this publication

Statistics

Seen <100 times