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Complete genome sequence of Menghai flavivirus, a novel insect-specific flavivirus from China

Authors
  • Zhang, Xianglilan1
  • Guo, Xiaofang2
  • Fan, Hang1
  • Zhao, Qiumin1
  • Zuo, Shuqing1
  • Sun, Qiang1
  • Pei, Guangqian1
  • Cheng, Shi1
  • An, Xiaoping1
  • Wang, Yunfei1
  • Mi, Zhiqiang1
  • Huang, Yong1
  • Zhang, Zhiyi1
  • Tong, Yigang1
  • Zhou, Hongning2
  • Zhang, Jiusong1
  • 1 Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogen and Biosecurity, Beijing, 100071, China , Beijing (China)
  • 2 Yunnan Institute of Parasitic Diseases, Yunnan Provincial Key Laboratory of Vector-borne Diseases Control and Research, Pu’er, Yunnan, 665000, China , Pu’er (China)
Type
Published Article
Journal
Archives of Virology
Publisher
Springer-Verlag
Publication Date
Feb 07, 2017
Volume
162
Issue
5
Pages
1435–1439
Identifiers
DOI: 10.1007/s00705-017-3232-5
Source
Springer Nature
Keywords
License
Yellow

Abstract

Menghai flavivirus (MFV) was isolated from Aedes albopictus in Menghai county of Yunnan Province, China, during an arboviruses screening program in August 2010. Whole genome sequencing of MFV was performed using an Ion PGM™ Sequencer. The complete genome of MFV was 10897 nucleotides in length and encoded a polyprotein and fairly interesting flavivirus orf (FIFO). The polyprotein contained three flavivirus structural proteins (C, prM/M and E) and seven nonstructural proteins. Nucleotide BLAST analysis revealed that the MFV genome showed highest similarity to Xishuangbanna Aedes flavivirus, a novel insect-specific flavivirus recently isolated from the same area. These species shared a query cover of 99%, but only 71% identity, while FIFO showed no similarity with any of the published sequences. Genomic and phylogenetic analyses suggested that MFV was a novel species of the genus Flavivirus. Our findings enrich our understanding of the genetics and prevalence of the family Flaviviridae.

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