Affordable Access

Access to the full text

Complete genome sequence of a distinct calla lily chlorotic spot virus isolated in mainland China

Authors
  • Xu, Ye1
  • Wang, Si-bo2
  • Li, Yong-zhong3
  • Tao, Hong-zheng4
  • Huang, Ya-ning2
  • Wu, Bao-wei2
  • Dong, Yu-mei2
  • Hu, John5
  • Liu, Ya-ting2
  • 1 Yunnan Agricultural University, College of Plant Protection, Kunming, 650201, China , Kunming (China)
  • 2 Yunnan Agricultural University, Center of Crop Science Experimental Teaching, College of Agronomy and Biotechnology, Kunming, 650201, China , Kunming (China)
  • 3 Yunnan Vocational and Technical College of Agriculture, Kunming, 650201, China , Kunming (China)
  • 4 Honghe University, College of Life Science and Technology, Mengzi, 661199, China , Mengzi (China)
  • 5 University of Hawaii, College of Tropical Agriculture and Human Resources, Honolulu, HI, 96822, USA , Honolulu (United States)
Type
Published Article
Journal
Archives of Virology
Publisher
Springer-Verlag
Publication Date
Oct 23, 2015
Volume
161
Issue
1
Pages
219–222
Identifiers
DOI: 10.1007/s00705-015-2630-9
Source
Springer Nature
Keywords
License
Yellow

Abstract

The first complete genome sequence of calla lily chlorotic spot virus (CCSV) from Lijiang in northwestern Yunnan Province was obtained using RT-PCR with designed primers. The genome of CCSV isolate LJ-1-Yunnan is tripartite. The small (S) RNA is 3182 nucleotides (nt) in length and encodes a nonstructural protein (NSs, 1383 nt) and a nuclear nucleocapsid (N, 834 nt), separated by an 836-nt intergenic region (IGR). The medium (M) RNA is 4749 nt in length and encodes a nonstructural movement protein (NSm, 930 nt) and a glycoprotein (GnGc, 3,372 nt), also separated by a 349-nt IGR. The large (L) RNA is 8912 nt in length and encodes a predicted RNA-dependent RNA polymerase (RdRp, 8652 nt). The nucleotide sequences of the three viral RNA segments are 92-94 % identical to the published CCSV genome sequence, and the amino acid sequences of the encoded proteins are 96-98 % identical. However, the IGRs of the S and M RNAs are less similar, with 86 and 72 % identity, respectively. Genome sequence comparisons and phylogenetic analysis indicate that the Lijiang CCSV isolate is a unique tospovirus isolate that differs from CCSV isolates in other geographic regions.

Report this publication

Statistics

Seen <100 times