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Complete cDNA sequence and mRNA expression of dog preproendothelin-3.

Authors
  • Fujimori, Yuki
  • Uchide, Tsuyoshi
  • Temma, Kyosuke
  • Sasaki, Takushi
  • Kizaki, Keiichiro
  • Hara, Yukio
  • Saida, Kaname
Type
Published Article
Journal
The Journal of veterinary medical science / the Japanese Society of Veterinary Science
Publication Date
Oct 01, 2004
Volume
66
Issue
10
Pages
1251–1254
Identifiers
PMID: 15528858
Source
Medline
License
Unknown

Abstract

The full-length cDNA of dog preproendothelin-3 (PPET3) was cloned from lung tissue using RT-PCR and rapid amplification of cDNA ends. Aside from the poly (A) tail, the full-length cDNA was 1976 bp. A polyadenylation signal sequence and one copy of a consensus sequence, ATTTA, which is related to mRNA turnover, was found in the 3' noncoding region. The cDNA had a 594-bp open reading frame encoding a 198-amino acid polypeptide. Regions corresponding to a bioactive mature ET3 peptide, an intermediate form known as big-ET3, and an ET3-like peptide were observed in dog PPET3. Expression of PPET3 mRNA was detected throughout the organs examined, which included heart, lung, liver, kidney, spleen, stomach, pancreas, duodenum, colon, uterus, ovary and testis.

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