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Competitive adsorption on gold nanoparticles for human papillomavirus 16 L1 protein detection by LDI-MS.

Authors
  • Zhu, Li1
  • Han, Jing2
  • Wang, Zhihua3
  • Yin, Lihui4
  • Zhang, Wei4
  • Peng, You5
  • Nie, Zongxiu2
  • 1 State Key Laboratory of Chemical Resource Engineering, and Beijing Advanced Innovation Center for Soft Matter Science and Engineering, Beijing University of Chemical Technology, Beijing 100029, China. [email protected] and National Institutes for Food and Drug Control, Beijing 102629, China. , (China)
  • 2 Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China. [email protected] , (China)
  • 3 State Key Laboratory of Chemical Resource Engineering, and Beijing Advanced Innovation Center for Soft Matter Science and Engineering, Beijing University of Chemical Technology, Beijing 100029, China. [email protected] , (China)
  • 4 National Institutes for Food and Drug Control, Beijing 102629, China. , (China)
  • 5 Department of Chemistry and Environment Engineering, Jiujiang University, Jiujiang, 332005 China. , (China)
Type
Published Article
Journal
The Analyst
Publisher
The Royal Society of Chemistry
Publication Date
Nov 21, 2019
Volume
144
Issue
22
Pages
6641–6646
Identifiers
DOI: 10.1039/c9an01612k
PMID: 31595888
Source
Medline
Language
English
License
Unknown

Abstract

The detection of the HPV L1 protein provides information about the infection status of the virus, reflects the replication status of the HPV virus in cervical cells, and helps understand the regression and progress of cervical lesions. Herein, we report a novel laser desorption ionization mass spectrometry (LDI MS) method for the sensitive detection of the HPV 16 L1 protein, based on non-covalent competitive adsorption between the HPV 16 L1 aptamer and melamine on gold nanoparticles (AuNPs). The intensity of the MS signal corresponding to the mass tag shows a linear relationship with the HPV 16 L1 concentration in the range 2-80 ng mL-1, with a limit of detection (LOD) of 58.8 pg mL-1. Using this method, the HPV 16 L1 protein is quantitatively analyzed in both clinical and vaccine samples. The described method is simple and has high sensitivity and good reliability.

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