Affordable Access

Access to the full text

Comparisons of urea or ammonium on growth and fermentative metabolism of Saccharomyces cerevisiae in ethanol fermentation

Authors
  • Yang, Xinchao1
  • Yang, Yuling2
  • Huang, Jiadong1
  • Man, Deen2
  • Guo, Maihai2
  • 1 University of Jinan, Jinan, 250022, China , Jinan (China)
  • 2 Linghua Group Limited, Jining, 272073, China , Jining (China)
Type
Published Article
Journal
World Journal of Microbiology and Biotechnology
Publisher
Springer Netherlands
Publication Date
May 10, 2021
Volume
37
Issue
6
Identifiers
DOI: 10.1007/s11274-021-03056-9
Source
Springer Nature
Keywords
License
Yellow

Abstract

This work was mainly about the understanding of how urea and ammonium affect growth, glucose consumption and ethanol production of S. cerevisiae, in particular regarding the basic physiology of cell. The basic physiology of cell included intracellular pH, ATP, NADH and enzyme activity. Results showed that fermentation time was reduced by 19% when using urea compared with ammonium. The maximal ethanol production rate using urea was 1.14 g/L/h, increasing 30% comparing with the medium prepared with ammonium. Moreover, urea could decrease the synthesis of glycerol from glucose by 26% comparing with ammonium. The by-product of acetic acid yields decreased from 40 mmol/mol of glucose (with urea) to 24 mmol/mol of glucose (with ammonium). At the end of ethanol fermentation, cell number and pH were greater with urea than ammonium. Comparing with urea, ammonium decreased the intracellular pH by 14% (from 7.1 to 6.1). Urease converting urea into ammonia resulted in a more than 50% lower of ATP when comparing with ammonium. The values of NADH/DCW were 0.21 mg/g and 0.14 mg/g respectively with urea and ammonium, suggesting a 33% lower NADH. The enzyme activity of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was 0.0225 and 0.0275 U/mg protein respectively with urea and ammonium, which was consistent with the yields of glycerol.

Report this publication

Statistics

Seen <100 times