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Comparison of specific blue and green fluorescence in cataractous versus normal human lens fractions.

Authors
Type
Published Article
Journal
Investigative Ophthalmology & Visual Science
0146-0404
Publisher
Association for Research in Vision and Opthalmology
Publication Date
Volume
34
Issue
3
Pages
630–636
Identifiers
PMID: 8449681
Source
Medline

Abstract

The similarity in blue fluorescence levels suggests that the blue fluorophore(s), although increasing in concentration with age, are not indicators of cataractogenesis and may not play an active role in opacification. The levels of specific green fluorescence indicate either a dramatic increase in the fluorescence quantum efficiency and/or an increase in the number of fluorescent sites per protein unit. The green-to-blue ratios were greater by a factor of as much as six in all cataractous soluble fractions versus clear ones. This suggests an accelerated formation of the green fluorescent species in cataractous tissues.

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