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Comparison of seven methods for DNA extraction from prosomata of the acorn barnacle, Amphibalanus amphitrite.

Authors
  • Schultzhaus, Janna N1
  • Taitt, Chris R2
  • Orihuela, Beatriz3
  • Smerchansky, Madeline4
  • Schultzhaus, Zachary S1
  • Rittschof, Daniel3
  • Wahl, Kathryn J5
  • Spillmann, Christopher M6
  • 1 National Research Council Research Associateship Program, Washington, DC, 20001, USA.
  • 2 Center for Bio/Molecular Science and Engineering, Naval Research Laboratory, Washington, DC, 20375, USA.
  • 3 Duke University Marine Laboratory, Beaufort, NC, 28516, USA.
  • 4 Naval Research Enterprise Internship Program, American Society for Engineering Education, 1818 N St. NW, Washington, DC, 20036, USA.
  • 5 Chemistry Division, Naval Research Laboratory, Washington, DC, 20375, USA.
  • 6 Center for Bio/Molecular Science and Engineering, Naval Research Laboratory, Washington, DC, 20375, USA. Electronic address: [email protected]
Type
Published Article
Journal
Analytical Biochemistry
Publisher
Elsevier
Publication Date
Dec 01, 2019
Volume
586
Pages
113441–113441
Identifiers
DOI: 10.1016/j.ab.2019.113441
PMID: 31539523
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Next generation sequencing (NGS) technologies can provide an understanding of the molecular processes involved in marine fouling by Amphibalanus spp. barnacles. Here, seven methods for extracting DNA from A. amphitrite prosomata were assessed with respect to recovery, purity and size distribution. Methods incorporating organic extractions generally resulted in low recovery of fragmented DNA. The most promising method was the commercial E.Z.N.A. Blood DNA Mini kit, which provided tens of micrograms of DNA of sufficient molecular weight for use in long-read NGS library preparation. Other kits resulted in DNA preps suitable for short read length NGS platforms. Copyright © 2019 Elsevier Inc. All rights reserved.

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