Comparison of seven methods for DNA extraction from prosomata of the acorn barnacle, Amphibalanus amphitrite.
National Research Council Research Associateship Program, Washington, DC, 20001, USA.
Center for Bio/Molecular Science and Engineering, Naval Research Laboratory, Washington, DC, 20375, USA.
Duke University Marine Laboratory, Beaufort, NC, 28516, USA.
Naval Research Enterprise Internship Program, American Society for Engineering Education, 1818 N St. NW, Washington, DC, 20036, USA.
Chemistry Division, Naval Research Laboratory, Washington, DC, 20375, USA.
Center for Bio/Molecular Science and Engineering, Naval Research Laboratory, Washington, DC, 20375, USA. Electronic address: [email protected]
- Published Article
- Publication Date
Dec 01, 2019
Next generation sequencing (NGS) technologies can provide an understanding of the molecular processes involved in marine fouling by Amphibalanus spp. barnacles. Here, seven methods for extracting DNA from A. amphitrite prosomata were assessed with respect to recovery, purity and size distribution. Methods incorporating organic extractions generally resulted in low recovery of fragmented DNA. The most promising method was the commercial E.Z.N.A. Blood DNA Mini kit, which provided tens of micrograms of DNA of sufficient molecular weight for use in long-read NGS library preparation. Other kits resulted in DNA preps suitable for short read length NGS platforms. Copyright © 2019 Elsevier Inc. All rights reserved.
Report this publication
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
This record was last updated on 12/31/2019 and may not reflect the most current and accurate biomedical/scientific data available from NLM.
The corresponding record at NLM can be accessed at https://www.ncbi.nlm.nih.gov/pubmed/31539523