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Comparison of rapid methods for analysis of bacterial fatty acids.

Authors
  • Moss, C W
  • Lambert, M A
  • Merwin, W H
Type
Published Article
Journal
Applied microbiology
Publication Date
Jul 01, 1974
Volume
28
Issue
1
Pages
80–85
Identifiers
PMID: 4844271
Source
Medline
License
Unknown

Abstract

When rapid gas-liquid chromatography methods for determination of bacterial fatty acids were compared, results showed that saponification was required for total fatty acid analysis. Transesterification with boron-trihalide reagents (BF(3)-CH(3)OH, BCl(3)-CH(3)OH) caused extensive degradation of cyclopropane acids and was less effective than saponification in releasing cellular hydroxy fatty acids. Digestion of cells with tetramethylammonium hydroxide was unsatisfactory because of extraneous gas-liquid chromatography peaks and because of lower recovery of branched-chain and hydroxy fatty acids. A simple, rapid saponification procedure which can be used for total cellular fatty acid analysis of freshly grown cells is described.

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