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Comparison of rapid DNA extraction methods applied to PCR identification of medicinal mushroom Ganoderma spp.

Authors
  • Zhou, Xuanwei
  • Li, Qizhang
  • Zhao, Jingya
  • Tang, Kexuan
  • Lin, Juan
  • Yin, Yizhou
Type
Published Article
Journal
Preparative biochemistry & biotechnology
Publication Date
Jan 01, 2007
Volume
37
Issue
4
Pages
369–380
Identifiers
PMID: 17849291
Source
Medline
License
Unknown

Abstract

Four different DNA extraction methods were used to extract genomic DNA of the medicinal mushroom Lingzhi from its developing stage materials, such as mycelium, dry fruiting body, or sliced and spore powder or sporoderm-broken spore powder. The DNA samples were analyzed using agarose gel electrophoresis, UV spectrophotometer, and PCR amplification. According to the average yields and purity of DNA, high salt concentrations and low pH methods were the best for DNA extraction. The mycelia and sporoderm-broken spore powder yielded higher and purer DNA. The method developed could effectively eliminate the influence of the secondary metabolites to DNA extraction. The DNA samples extracted from the developed method could be successfully used for PCR applications.

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