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Comparison of polymerase chain reaction assays with bacteriologic culture, immunofluorescence, and nucleic acid hybridization for detection of Leptospira borgpetersenii serovar hardjo in urine of cattle.

Authors
  • Wagenaar, J
  • Zuerner, R L
  • Alt, D
  • Bolin, C A
Type
Published Article
Journal
American Journal of Veterinary Research
Publisher
American Veterinary Medical Association
Publication Date
Mar 01, 2000
Volume
61
Issue
3
Pages
316–320
Identifiers
PMID: 10714525
Source
Medline
License
Unknown

Abstract

Bacteriologic culture, PCR, and immunofluorescence were sensitive for detection of L borgpetersenii serovar hardjo type hardjo-bovis in urine specimens of cattle, but a single technique was not the most sensitive for each animal tested. Therefore, the use of 2 techniques in combination is warranted for maximal sensitivity for diagnosis.

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