Affordable Access

deepdyve-link
Publisher Website

Comparison of Placenta PCR and Maternal Serology of Aborted Women for Detection of Toxoplasma gondii in Ardabil, Iran.

Authors
  • Matin, Somaie1
  • Shahbazi, Gholamreza2
  • Namin, Shervin Tabrizian3
  • Moradpour, Rouhallah4
  • Feizi, Farideh4
  • Piri-Dogahe, Hadi5
  • 1 Department of Internal Medicine, Ardabil University of Medical Sciences, Ardabil, Iran. , (Iran)
  • 2 Department of Parasitology and Mycology, Faculty of Specialized Veterinary Science, Islamic Azad University, Science and Research Branch, Tehran, Iran. , (Iran)
  • 3 Department of Obstetrics and Gynecological, Ardabil University of Medical Sciences, Ardabil, Iran. , (Iran)
  • 4 Ardabil University of Medical Sciences, Ardabil, Iran. , (Iran)
  • 5 Department of Microbiology, Ardabil University of Medical Sciences, Ardabil, Iran. , (Iran)
Type
Published Article
Journal
The Korean journal of parasitology
Publication Date
Dec 01, 2017
Volume
55
Issue
6
Pages
607–611
Identifiers
DOI: 10.3347/kjp.2017.55.6.607
PMID: 29320815
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Primary maternal infection with toxoplasmosis during pregnancy is frequently associated with transplacental transmission of the parasite to the fetus. This study was conducted to test the utility of PCR assay to detect recent infections with Toxoplasma in aborted women at various gestational ages who referred to Obstetrics and Gynecology Department of Alavi Hospital in Ardabil during 2014 and 2016. Two hundred women with a history of single or repeated abortion were investigated in this study. Blood samples were tested for specific anti-Toxoplasma IgM and IgG antibodies by ELISA. According to the results, 53.5% of the women under study were positive for anti-Toxoplasma antibodies: 4.0% of them had IgM, 43.0% had IgG, and 6.5% had both IgM and IgG. Subsequently, Nested-PCR analysis was used to detect T. gondii DNA in the placenta of subjects. In 10.5% of the women, the results were positive for 529 bp element of T. gondii. Among them, 5 (23.8%) cases were IgM positive, 1 (4.8%) case was IgG positive, and 11 (52.4%) were both IgM and IgG positive. In 4 (19.0%) patients, none of the antibodies were found to be positive. In total, 16 patients had positive results in both ELISA and PCR methods, and 174 cases had negative results for new infection. The findings of this study revealed that T. gondii might be one of the significant factors leading to abortion, and that the analysis of placenta can be important in order to achieve increased detection sensitivity.

Report this publication

Statistics

Seen <100 times