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Comparison of mRNA expression levels determined with TaqMan and competitive template RT-PCR.

Authors
  • Mauritz, R
  • Schwabe, W
  • Haeusler, P
  • Noordhuis, P
  • Smid, K
  • McLeod, H L
  • Behnke, D
  • van Groeningen, C J
  • Peters, G J
Type
Published Article
Journal
Nucleosides, nucleotides & nucleic acids
Publication Date
Oct 01, 2004
Volume
23
Issue
8-9
Pages
1471–1474
Identifiers
PMID: 15571279
Source
Medline
License
Unknown

Abstract

Two methods for measurement of thymidylate synthase (TS) and dihydropyrimidine dehydrogenase (DPD) mRNA expression were compared. Although the relative mRNA levels compared to beta-actin measured with competitive template RT-PCR were different from the data obtained with a TaqMan based PCR, a significant correlation between the two assays was found.

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