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Comparison of microbiota and allergen profile in house dust from homes of allergic and non-allergic subjects- results from the GUSTO study

Authors
  • Loo, Evelyn Xiu Ling1
  • Chew, Lamony Jian Ming2
  • Zulkifli, Atiqa Binte2
  • Ta, Le Duc Huy2
  • Kuo, I-Chun2
  • Goh, Anne3
  • Teoh, Oon Hoe4
  • Van Bever, Hugo2, 5
  • Gluckman, Peter D.6, 7
  • Yap, Fabian8
  • Tan, Kok Hian9
  • Chong, Yap Seng1, 10
  • Lee, Bee Wah2
  • Shek, Lynette Pei-chi1, 2, 5
  • 1 Singapore Institute for Clinical Sciences (SICS), Agency for Science, Technology and Research (A*STAR), Singapore, Singapore , Singapore (Singapore)
  • 2 Yong Loo Lin School of Medicine, National University of Singapore, Department of Paediatrics, Singapore, Singapore , Singapore (Singapore)
  • 3 KK Women’s and Children’s Hospital, Allergy Service, Department of Paediatrics, Singapore, Singapore , Singapore (Singapore)
  • 4 KK Women’s and Children’s Hospital, Respiratory Medicine Service, Department of Paediatrics, Singapore, Singapore , Singapore (Singapore)
  • 5 National University Hospital, National University Health System, Khoo Teck Puat-National University Children’s Medical Institute, Singapore, Singapore , Singapore (Singapore)
  • 6 Growth, Development and Metabolism Programme, Singapore Institute for Clinical Sciences (SICS), Agency for Science, Technology and Research (A*STAR), Singapore, Singapore , Singapore (Singapore)
  • 7 University of Auckland, Liggins Institute, Auckland, New Zealand , Auckland (New Zealand)
  • 8 KK Women’s and Children’s Hospital, Department of Endocrinology, Singapore, Singapore , Singapore (Singapore)
  • 9 KK Women’s and Children’s Hospital, Department of Maternal Fetal Medicine, Singapore, Singapore , Singapore (Singapore)
  • 10 Yong Loo Lin School of Medicine, National University of Singapore, Department of Obstetrics & Gynaecology, Singapore, Singapore , Singapore (Singapore)
Type
Published Article
Journal
World Allergy Organization Journal
Publisher
BioMed Central
Publication Date
Dec 05, 2018
Volume
11
Issue
1
Identifiers
DOI: 10.1186/s40413-018-0212-5
Source
Springer Nature
License
Green

Abstract

BackgroundThe prevalence of allergic diseases, such as asthma, allergic rhinitis, eczema and food allergy, has been increasing worldwide, as shown in a large number of studies, including the International Study of Asthma and Allergies in Childhood (ISAAC). However, there is significant variation in the prevalence of these diseases in different regions, suggesting that there may be location-specific factors such as environment and microbial exposure affecting allergic disease prevalence. Hence, in this study we determine if there is a difference in microbiota composition and allergen concentration of household dust collected from the homes of non-allergic and allergic subjects from the Growing Up in Singapore Towards Healthy Outcomes (GUSTO) cohort.MethodsFrom the Growing Up in Singapore Towards Healthy Outcomes (GUSTO) cohort, 25 allergic subjects and 25 non-allergic subjects were selected at the year 5.5 follow up. Definitions of allergic outcomes were standardized in the questionnaires administered at 3, 6, 9, 12, 15, 18, 24, 36, 48 and 60 months to ensure consistency during interviews and home visits. Allergen sensitization was determined by skin prick testing (SPT) at 18, 36 and 60 months. Dust samples were collected from the subject’s bed, sofa, and play area. DNA extraction was carried out and V3-V4 hypervariable regions of bacterial 16S rRNA gene were sequenced. Protein extraction was performed and allergens assayed by using multiplex assay and ELISA.ResultsThe most abundant phyla in house dust were Actinobacteria (29.8%), Firmicutes (27.7%), and Proteobacteria (22.4%). Although there were no differences in bacteria abundance and diversity between house dust samples of allergic and non-allergic subjects, the relative abundance of Anaplasmataceae, Bacteroidaceae, and Leptospiraceae were significantly higher in dust samples of allergic subjects as compared to non-allergic subjects in 2 or more locations. The concentration of Der p 1 was significantly lower in bed dust samples of allergic subjects (Median [Interquartile range], 174 ng/g [115–299 ng/g]) as compared to non-allergic subjects (309 ng/g [201–400 ng/g]; P < 0.05). The concentration of tropomyosin was significantly higher in sofa dust samples of allergic subjects (175 ng/g [145–284 ng/g] as compared to non-allergic subjects (116 ng/g [52.8–170 ng/g]; P < 0.05).ConclusionIn conclusion, we found a differential microbiota and allergen profile between homes of allergic and non-allergic subjects.Trial registrationNCT01174875 Registered 1 July 2010, retrospectively registered.

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