Affordable Access

deepdyve-link
Publisher Website

Comparison of Different Decalcification Methods Using Rat Mandibles as a Model.

Authors
  • Savi, Flavia M1
  • Brierly, Gary I1
  • Baldwin, Jeremy1
  • Theodoropoulos, Christina1
  • Woodruff, Maria A1
  • 1 Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Queensland, Australia. , (Australia)
Type
Published Article
Journal
Journal of Histochemistry & Cytochemistry
Publisher
SAGE Publications
Publication Date
Dec 01, 2017
Volume
65
Issue
12
Pages
705–722
Identifiers
DOI: 10.1369/0022155417733708
PMID: 28958188
Source
Medline
Keywords
License
Unknown

Abstract

Selection of decalcification agents is an essential consideration when processing mineralized tissues because the integrity and immunohistochemical characteristics of the tissues may be affected. Here, we report results obtained from the decalcification of rat mandibles using 10% ethylenediaminetetraacetic acid (EDTA) at room temperature (RT), 10% EDTA at 37C, 5% nitric acid, and 10% formic acid at RT. Decalcification endpoints were determined by microcomputed tomography. Morphological preservation and antigenicity were evaluated by hematoxylin and eosin staining and immunohistochemistry. Decalcification of the anterior and posterior portions of the mandible took 220 and 191 hr in 10% EDTA RT, 102 and 73 hr in 10% EDTA 37C, 13.5 and 4.3 hr in 5% nitric acid, and 140 and 36 hr in 10% formic acid, respectively. Decalcification in 10% EDTA at 37C was accelerated, but 10% EDTA at RT provided optimal results for immunohistochemistry and cellular and structural details. Decalcification using 5% nitric acid was accomplished in the shortest time and exhibited good cellular and architectural morphology, whereas 10% formic acid was suboptimal with respect to tissue and cellular morphology. Despite being the slowest method, EDTA at RT is still the recommended method for decalcifying mineralized tissues; however, if rapid decalcification is needed, 5% nitric acid is the best option, yielding acceptable tissue integrity and speed.

Report this publication

Statistics

Seen <100 times