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Comparison of the binding of nicotinic agonists to receptors from human and rat cerebral cortex and from chick brain (alpha 4 beta 2) transfected into mouse fibroblasts with ion channel activity.

Authors
  • Court, J A
  • Perry, E K
  • Spurden, D
  • Lloyd, S
  • Gillespie, J I
  • Whiting, P
  • Barlow, R
Type
Published Article
Journal
Brain Research
Publisher
Elsevier
Publication Date
Dec 19, 1994
Volume
667
Issue
1
Pages
118–122
Identifiers
PMID: 7534607
Source
Medline
License
Unknown

Abstract

(-)-Nicotine, cytisine and carbachol evoked 86Rb efflux from mouse fibroblasts stably transfected with alpha 4 beta 2 chick brain nicotinic subunits. This response to (-)-nicotine was inhibited by mecamylamine and dihydro-beta-erythroidine and was mirrored by a rise in intracellular Ca2+ measured by microspectrofluorimetry. Lobeline and isoarecolone methiodide evoked no significant 86Rb from cells and unlike the above agonists displayed significantly different IC50 values for the displacement of [3H]nicotine from mammalian (rat and human cerebral cortex) and transfected fibroblast membranes.

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