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Comparison of automated ribotyping to pulsed-field gel electrophoresis for genetic fingerprinting of Streptococcus pneumoniae.

Authors
  • Quale, J M
  • Landman, D
  • Flores, C
  • Ravishankar, J
Type
Published Article
Journal
Journal of clinical microbiology
Publication Date
Nov 01, 2001
Volume
39
Issue
11
Pages
4175–4177
Identifiers
PMID: 11682553
Source
Medline
License
Unknown

Abstract

Fifty-two isolates of Streptococcus pneumoniae were characterized by pulsed-field gel electrophoresis (PFGE) and automated ribotyping by using HindIII and PvuII. HindIII ribotypes correlated well with PFGE. PvuII produced fewer bands and was less discriminatory. Automated ribotyping with HindIII is an accurate method for genetic fingerprinting of S. pneumoniae and can complement PFGE.

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