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Comparison of anticarcinogenic properties of Viburnum opulus and its active compound p-coumaric acid on human colorectal carcinoma.

Authors
  • Karakurt, Serdar1
  • AbuŞoĞlu, Gülsüm2
  • Arituluk, Zekiye Ceren3
  • 1 Department of Biochemistry, Faculty of Science, Selçuk University, Konya Turkey. , (Turkey)
  • 2 Department of Medical Laboratory Techniques, Vocational School of Health, Selçuk University, Konya Turkey. , (Turkey)
  • 3 Department of Pharmaceutical Botany, Faculty of Pharmacy, Hacettepe University, Ankara Turkey. , (Turkey)
Type
Published Article
Journal
Turkish journal of biology = Turk biyoloji dergisi
Publication Date
Jan 01, 2020
Volume
44
Issue
5
Pages
252–263
Identifiers
DOI: 10.3906/biy-2002-30
PMID: 33110363
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Resistance to therapeutic agents and the highly toxic side effects of synthetic drugs has spurred new research in the treatment of colon cancer, which has high morbidity and mortality ratios. This study aims to clarify the molecular mechanisms of the anticarcinogenic properties of methanol extract of Viburnum opulus L. (EVO)and its main active compound, trans-p -coumaric acid ( p -CA), on human colon cancer cells (DLD-1, HT-29, SW-620, Caco-2) and healthy colon epithelial cells (CCD-18Co). The effects of EVO on controlled cell death (apoptosis) and the cell division cycle were determined by flow cytometry. Alteration in mRNA and protein expressions of switch genes in colorectal carcinoma (APC, MLH1, TP53, SMAD4, KRAS, and BRAF) were determined by qRT-PCR and Western blot, respectively. Our results show that EVO possesses a strong reducing capacity and free-radical scavenging activity. HPLC analyses prove that p -CAis the main compound of EVO. EVO and p -CA inhibit the proliferation of human colon cancer cells DLD-1 and HT-29 in a dose-dependent manner. EVO increases apoptosis of DLD-1 cells and halts the cell cycle in the G2 stage in HT-29 cells. mRNA and protein expressions of p53 and SMAD-4 are upregulated, while BRAFs are downregulated. The results were directly proportional to p -CA. EVO and p -CA up- and downregulate switch genes and protein expressions of DLD-1 cells, which alter the expression of 186 other genes. This is the first study of pharmacological exploration of V.opulus in human colon cancer. Its antiproliferative effects may be due to the presence of p -CA. Copyright © 2020 The Author(s).

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