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Comparative transcriptome analysis of two citrus germplasms with contrasting susceptibility to Phytophthora nicotianae provides new insights into tolerance mechanisms

Authors
  • Ajengui, Arwa1, 2, 3
  • Bertolini, Edoardo4
  • Ligorio, Angela2
  • Chebil, Samir1
  • Ippolito, Antonio2
  • Sanzani, Simona Marianna2
  • 1 Center of Biotechnology of Borj-Cédria, Laboratory of Plant Molecular Physiology, Hammam-Lif, 2050, Tunisia , Hammam-Lif (Tunisia)
  • 2 Università degli Studi di Bari “Aldo Moro”, Dipartimento di Scienze del Suolo, della Pianta e degli Alimenti, Via Amendola 165/A, Bari, 70126, Italy , Bari (Italy)
  • 3 Université Tunis El Manar, Faculté des Sciences de Tunis, LR03ES03 Laboratoire Microorganismes et Biomolécules Actives, Tunis, 2092, Tunisia , Tunis (Tunisia)
  • 4 Scuola Superiore Sant’Anna, Institute of Life Sciences, Piazza Martiri della Libertà 33, Pisa, 56127, Italy , Pisa (Italy)
Type
Published Article
Journal
Plant Cell Reports
Publisher
Springer-Verlag
Publication Date
Dec 30, 2017
Volume
37
Issue
3
Pages
483–499
Identifiers
DOI: 10.1007/s00299-017-2244-7
Source
Springer Nature
Keywords
License
Yellow

Abstract

Key messageHost perception of Phytophthora nicotianae switching to necrotrophy is fundamental for disease tolerance of citrus. It involves an HR-like response, strengthening of the cell wall structure and hormonal signaling.AbstractStem rot caused by P. nicotianae is a worldwide disease of several important crops, including citrus. Given the growing awareness of chemical fungicides drawbacks, genetic improvement of citrus rootstocks remains the best alternative. However, the molecular basis underlying the successful response of resistant and/or tolerant genotypes remains poorly understood. Therefore, we performed a transcriptomic analysis to examine the differential defense response to P. nicotianae of two germplasms—tolerant sour orange (SO, Citrus aurantium) and susceptible Madam Vinous (MV, C. sinensis)—in both the biotrophic and necrotrophic phases of host–pathogen interaction. Our results revealed the necrotrophic phase as a decisive turning point, since it included stronger modulation of a number of genes implicated in pathogen perception, signal transduction, HR-like response, transcriptional reprogramming, hormone signaling, and cell wall modifications. In particular, the pathogen perception category reflected the ability of SO to perceive the pathogen even after its switch to necrotrophy, and thus to cope successfully with the infection, while MV failed. The concomitant changes in genes involved in the remaining functional categories seemed to prevent pathogen spread. This investigation provided further understanding of the successful defense mechanisms of C. aurantium against P. nicotianae, which might be exploited in post-genomic strategies to develop resistant Citrus genotypes.

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