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A comparative study of procedures for binding of aflatoxin M1 to Lactobacillus rhamnosus GG.

Authors
  • Assaf, Jean Claude1
  • Atoui, Ali2
  • Khoury, André El3
  • Chokr, Ali4
  • Louka, Nicolas3
  • 1 Centre d'Analyses et de Recherche (CAR), Unité de Recherche Technologies et Valorisation agro-Alimentaire (UR-TVA), Faculté des Sciences, Université Saint-Joseph de Beyrouth, Campus des Sciences et Technologies, Mar Roukos, Matn, Lebanon; Laboratory of Microbiology, Department of Life and Earth Sciences, Faculty of Sciences I Lebanese University Hadat Campus, Beirut, Lebanon; Platform of Research and Analysis in Environmental Sciences (PRASE), Doctoral School of Sciences and Technologies, Lebanese University, Hadat Campus, Beirut, Lebanon; Ecole Doctorale "Sciences et Santé", Université Saint-Joseph de Beyrouth, Campus des Sciences Médicales et Infirmières, Riad El Solh, Beyrouth, Lebanon. , (Lebanon)
  • 2 Laboratory of Microbiology, Department of Life and Earth Sciences, Faculty of Sciences I Lebanese University Hadat Campus, Beirut, Lebanon. , (Lebanon)
  • 3 Centre d'Analyses et de Recherche (CAR), Unité de Recherche Technologies et Valorisation agro-Alimentaire (UR-TVA), Faculté des Sciences, Université Saint-Joseph de Beyrouth, Campus des Sciences et Technologies, Mar Roukos, Matn, Lebanon. , (Lebanon)
  • 4 Laboratory of Microbiology, Department of Life and Earth Sciences, Faculty of Sciences I Lebanese University Hadat Campus, Beirut, Lebanon; Platform of Research and Analysis in Environmental Sciences (PRASE), Doctoral School of Sciences and Technologies, Lebanese University, Hadat Campus, Beirut, Lebanon. Electronic address: [email protected] , (Lebanon)
Type
Published Article
Journal
Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]
Publication Date
Aug 06, 2017
Identifiers
DOI: 10.1016/j.bjm.2017.05.003
PMID: 28843807
Source
Medline
Keywords
License
Unknown

Abstract

Several strains of lactic acid bacteria (LAB), frequently used in food fermentation and preservation, have been reported to bind different types of toxins in liquid media. This study was carried out to investigate the effect of different concentrations of Lactobacillus rhamnosus GG (ATCC 53103) to bind aflatoxin M1 (AFM1) in liquid media. AFM1 binding was tested following repetitive washes or filtration procedures in combination with additional treatments such as heating, pipetting, and centrifugation. The mixture of L. rhamnosus GG and AFM1 was incubated for 18h at 37°C and the binding efficiency was determined by quantifying the unbound AFM1 using HPLC. The stability of the complexes viable bacteria-AFM1 and heat treated bacteria-AFM1 was tested. Depending on the bacterial concentration and procedure used, the percentages of bound AFM1 by L. rhamnosus GG varied from as low as undetectable to as high as 63%. The highest reduction in the level of unbound AFM1 was recorded for the five washes procedure that involved heating and pipetting. Results also showed that binding was partially reversible and AFM1 was released after repeated washes. These findings highlight the effect of different treatments on the binding of AFM1 to L. rhamnosus GG in liquid matrix.

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