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A comparative study of fluorescent microscopy with Ziehl-Neelsen staining and culture for the diagnosis of pulmonary tuberculosis.

Authors
  • Laifangbam, S
  • Singh, H Lokhendro
  • Singh, N B
  • Devi, K M
  • Singh, N T
Type
Published Article
Journal
Kathmandu University medical journal (KUMJ)
Publication Date
Jan 01, 2009
Volume
7
Issue
27
Pages
226–230
Identifiers
PMID: 20071867
Source
Medline
License
Unknown

Abstract

The better case detection rates of AO over ZN were comparable to those found by several studies. Since screening was done under lower power of magnification (400x), fluorescence microscopy has been found to be less time consuming as compared to ZN method (1000x) in the diagnosis of tuberculosis. The tubercle bacilli stood out as bright objects against a dark background in fluorescence microscopy which makes them easily identifiable hence causing less eye-strain. The efficacy of fluorescence microscopy proved to be much higher than conventional light microscopy and comparable to that of culture.

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