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Comparative Analysis of UV Irradiation Effects on Escherichia coli and Pseudomonas aeruginosa Bacterial Cells Utilizing Biological and Computational Approaches.

Authors
  • Margaryan, A1, 2
  • Badalyan, H3
  • Trchounian, A4, 5
  • 1 Department of Microbiology, Plants and Microbes Biotechnology, Faculty of Biology, Yerevan State University, 1 Alex Manoogian Str, 0025, Yerevan, Armenia.
  • 2 Research Institute of Biology, Faculty of Biology, Yerevan State University, 1 Alex Manoogian Str, 0025, Yerevan, Armenia.
  • 3 Department of General Physics and Astrophysics, Faculty of Physics, Yerevan State University, 1 Alex Manoogian Str, 0025, Yerevan, Armenia.
  • 4 Department of Microbiology, Plants and Microbes Biotechnology, Faculty of Biology, Yerevan State University, 1 Alex Manoogian Str, 0025, Yerevan, Armenia. [email protected]
  • 5 Research Institute of Biology, Faculty of Biology, Yerevan State University, 1 Alex Manoogian Str, 0025, Yerevan, Armenia. [email protected]
Type
Published Article
Journal
Cell Biochemistry and Biophysics
Publisher
Springer-Verlag
Publication Date
September 2016
Volume
74
Issue
3
Pages
381–389
Identifiers
DOI: 10.1007/s12013-016-0748-3
PMID: 27334536
Source
Medline
Keywords
License
Unknown

Abstract

Microorganisms have a large number of tools to withstand different, and sometimes strong, environmental stresses, including irradiation, but this ability should be further evaluated for certain applications. Growth inhibition and morphological alterations of Escherichia coli M-17 and Pseudomonas aeruginosa GRP3 wild-type cells caused by UV-A irradiation have been detected in the present study. Comparative analysis was carried out using well-established microbiological methods (determination of specific growth rate, growth lag phase duration, and colony-forming unit number-CFU) and computational approaches, employing light microscopy and digital image analysis to evaluate bacterial cell morphology. Decreases in the specific growth rate, prolonged lag-phases, and lowered CFUs were observed after 5 and 10 min of UV irradiation (approx. 40 Gy) compared to the control (nonirradiated) cells. Accordingly, two computational parameters-the average bacterial cell surface area and the bacterial cell perimeter (i.e., of the 2D projection of bacterial cells in microscopy image)-were reduced. The ratio of bacterial cell surface area (S) to the square of the perimeter (p (2) ) was reduced after 5 min of irradiation, but after 10 min of irradiation the studied bacterial cells became flat cylinders. The revealed findings are concluded to be highly useful in developing new, rapid analysis methods to monitor environmental and UV irradiation effects on bacteria and to detect bacterial cell morphology alterations.

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