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Comparative Analysis of Pseudo-nitzschia Chloroplast Genomes Revealed Extensive Inverted Region Variation and Pseudo-nitzschia Speciation

Authors
  • He, Ziyan1, 2, 3, 4
  • Chen, Yang1, 2, 3, 4
  • Wang, Yichao1, 2, 3, 4
  • Liu, Kuiyan1, 2, 3, 4
  • Xu, Qing5
  • Li, Yang6
  • Chen, Nansheng1, 2, 4, 7
  • 1 Chinese Academy of Sciences (CAS) Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences (CAS), Qingdao , (China)
  • 2 Laboratory of Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao , (China)
  • 3 College of Marine Science, University of Chinese Academy of Sciences (CAS), Beijing , (China)
  • 4 Center for Ocean Mega-Science, Chinese Academy of Sciences (CAS), Qingdao , (China)
  • 5 College of Life Science and Technology, Huazhong Agricultural University, Wuhan , (China)
  • 6 Guangdong Provincial Key Laboratory of Healthy and Safe Aquaculture, College of Life Science, South China Normal University, Guangzhou , (China)
  • 7 Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, BC , (Canada)
Type
Published Article
Journal
Frontiers in Marine Science
Publisher
Frontiers Media S.A.
Publication Date
May 18, 2022
Volume
9
Identifiers
DOI: 10.3389/fmars.2022.784579
Source
Frontiers
Keywords
Disciplines
  • Marine Science
  • Original Research
License
Green

Abstract

Pseudo-nitzschia is a species-rich genus where many species can induce harmful algae blooms (HABs) associated with the toxin domoic acid (DA) production. Despite the importance of Pseudo-nitzschia species to coastal environments, their genomic information is rather limited, hindering research on biodiversity and evolutionary analysis. In this study, we report full-length chloroplast genomes (cpDNAs) of nine Pseudo‚Äźnitzschia, among which cpDNAs of eight Pseudo-nitzschia species were reported for the first time. The sizes of these Pseudo-nitzschia cpDNAs, which showed typical quadripartite structures, varied substantially, ranging from 116,546 bp to 158,840 bp in size. Comparative analysis revealed the loss of photosynthesis-related gene psaE in cpDNAs of all Pseudo-nitzschia species except that of P. americana, and the selective loss of rpl36 in P. hainanensis. Phylogenetic analysis showed that all Pseudo-nitzschia strains were grouped into two clades, with clade 1 containing cpDNAs of P. multiseries, P. pungens, P. multistriata, and P. americana, and clade 2 containing cpDNAs of P. hainanensis, P. cuspidata, Pseudo-nitzschia sp. CNS00097, P. delicatissima, and P. micropora. The small size of the P. americana cpDNA was primarily due to its shortened inverted repeat (IR) regions. While psaA and psaB were found in the IR regions of cpDNAs of other eight species, these two genes were found outside of the IR regions of P. americana cpDNA. In contrast, P. hainanensis had the largest size because of expansion of IR regions with each IR region containing 15 protein-coding genes (PCGs). Eleven genetic regions of these Pseudo-nitzschia cpDNAs exhibited high nucleotide diversity (Pi) values, suggesting that these regions may be used as molecular markers for distinguishing different Pseudo-nitzschia species with high resolution and high specificity. Phylogenetic analysis of the divergence of nine Pseudo-nitzschia species indicated that these species appeared at approximately 41 Mya. This study provides critical cpDNA resources for future research on the biodiversity and speciation of Pseudo-nitzschia species.

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