The ErgoKiTa study aimed to determine the musculoskeletal strain of preschool teachers and to identify and evaluate suitable prevention measures to reduce this strain. A comprehensive work analysis using objective and subjective methods was performed to determine the present work situation in preschools in Germany, and the results were used to derive suitable intervention measures. The musculoskeletal strain was determined by means of a comprehensive analysis of postures, forces and movements using the CUELA system and calculated as cumulative shift workloads. The intervention measures were evaluated in a pre- and post-intervention assessment for 12 participants. Significant alterations in the duration of postures were determined, specifically for the daily duration of knee-straining postures as well as the degree of trunk flexion between 60° and 90°, which were reduced from 8.4 to 3.1% and from 3.7 to 2.4%, respectively, following the intervention. Practitioner Summary: Research has shown that preschool teachers are at risk of developing musculoskeletal disorders. The effects of a situation-orientated and behaviour-orientated intervention approach were assessed with regard to awkward working postures. Significant alterations in the duration of postures following the intervention were found, specifically for knee-straining postures.