Affordable Access

deepdyve-link
Publisher Website

Common virulence factors for Pseudomonas tolaasii pathogenesis in Agaricus and Arabidopsis

Authors
  • Chung, In-Young
  • Kim, Young-Kee
  • Cho, You-Hee1, 2, 3, 4
  • 1 Department of Pharmacy
  • 2 CHA University
  • 3 Department of Environmental and Biological Chemistry
  • 4 Chungbuk National University
Type
Published Article
Journal
Research in Microbiology
Publisher
Elsevier
Publication Date
Jan 01, 2013
Accepted Date
Dec 03, 2013
Volume
165
Issue
2
Pages
102–109
Identifiers
DOI: 10.1016/j.resmic.2013.12.001
Source
Elsevier
Keywords
License
Unknown

Abstract

Brown blotch of cultivatable mushrooms is a disease caused by the small peptide toxin (tolaasin) secreted by Pseudomonas tolaasii. Here we found that the wild type tolassin-producing P. tolaasii stain 6264 was capable of infection in Arabidopsis thaliana cotyledons, causing chlorotic symptoms and growth arrest as a result of bacterial proliferation. Seven virulence-attenuated mutants of P. tolaasii were isolated from the Agaricus bisporus screen using 2512 mariner-based transposon insertion mutants, and all of them displayed reduced virulence and bacterial proliferation in Arabidopsis infection as well. The transposon was inserted within the genes for tolassin biosynthesis and amino acid biosynthesis, and within an intergenic region between the genes of unknown function. The finding that some virulence factors are commonly required for both Agaricus and Arabidopsis infections suggests that Arabidopsis could be exploited to study the host–pathogen interaction involving P. tolaasii.

Report this publication

Statistics

Seen <100 times