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Common and Rare Genetic Variants That Could Contribute to Severe Otitis Media in an Australian Aboriginal Population.

Authors
  • Jamieson, Sarra E1
  • Fakiola, Michaela2
  • Tang, Dave1
  • Scaman, Elizabeth1
  • Syn, Genevieve1
  • Francis, Richard W1
  • Coates, Harvey L3
  • Anderson, Denise1
  • Lassmann, Timo1
  • Cordell, Heather J4
  • Blackwell, Jenefer M1
  • 1 Telethon Kids Institute, The University of Western Australia, Perth, Western Australia. , (Australia)
  • 2 FIRC Institute of Molecular Oncology (IFOM), Milan, Italy. , (Italy)
  • 3 Faculty of Health and Medical Sciences, The University of Western Australia, Perth, Western Australia. , (Australia)
  • 4 Population Health Sciences Institute, Newcastle University, Newcastle upon Tyne, UK.
Type
Published Article
Journal
Clinical Infectious Diseases
Publisher
Oxford University Press
Publication Date
Nov 16, 2021
Volume
73
Issue
10
Pages
1860–1870
Identifiers
DOI: 10.1093/cid/ciab216
PMID: 33693626
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Our goal was to identify genetic risk factors for severe otitis media (OM) in Aboriginal Australians. Illumina® Omni2.5 BeadChip and imputed data were compared between 21 children with severe OM (multiple episodes chronic suppurative OM and/or perforations or tympanic sclerosis) and 370 individuals without this phenotype, followed by FUnctional Mapping and Annotation (FUMA). Exome data filtered for common (EXaC_all ≥ 0.1) putative deleterious variants influencing protein coding (CADD-scaled scores ≥15] were used to compare 15 severe OM cases with 9 mild cases (single episode of acute OM recorded over ≥3 consecutive years). Rare (ExAC_all ≤ 0.01) such variants were filtered for those present only in severe OM. Enrichr was used to determine enrichment of genes contributing to pathways/processes relevant to OM. FUMA analysis identified 2 plausible genetic risk loci for severe OM: NR3C1 (Pimputed_1000G = 3.62 × 10-6) encoding the glucocorticoid receptor, and NREP (Pimputed_1000G = 3.67 × 10-6) encoding neuronal regeneration-related protein. Exome analysis showed: (i) association of severe OM with variants influencing protein coding (CADD-scaled ≥ 15) in a gene-set (GRXCR1, CDH23, LRP2, FAT4, ARSA, EYA4) enriched for Mammalian Phenotype Level 4 abnormal hair cell stereociliary bundle morphology and related phenotypes; (ii) rare variants influencing protein coding only seen in severe OM provided gene-sets enriched for "abnormal ear" (LMNA, CDH23, LRP2, MYO7A, FGFR1), integrin interactions, transforming growth factor signaling, and cell projection phenotypes including hair cell stereociliary bundles and cilium assembly. This study highlights interacting genes and pathways related to cilium structure and function that may contribute to extreme susceptibility to OM in Aboriginal Australian children. © The Author(s) 2021. Published by Oxford University Press for the Infectious Diseases Society of America.

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