A common F-box gene regulates the leucine homeostasis of Medicago truncatula and Arabidopsis thaliana.
- Authors
- Type
- Published Article
- Journal
- PROTOPLASMA
- Publisher
- Springer-Verlag
- Publication Date
- Mar 01, 2022
- Volume
- 259
- Issue
- 2
- Pages
- 277–290
- Identifiers
- DOI: 10.1007/s00709-021-01662-w
- PMID: 33973099
- Source
- Medline
- Keywords
- Language
- English
- License
- Unknown
Abstract
The F-box domain is a conserved structural protein motif that most frequently interacts with the SKP1 protein, the core of the SCFs (SKP1-CULLIN-F-box protein ligase) E3 ubiquitin protein ligases. As part of the SCF complexes, the various F-box proteins recruit substrates for degradation through ubiquitination. In this study, we functionally characterized an F-box gene (MtF-box) identified earlier in a population of Tnt1 retrotransposon-tagged mutants of Medicago truncatula and its Arabidopsis thaliana homolog (AtF-box) using gain- and loss-of-function plants. We highlighted the importance of MtF-box in leaf development of M. truncatula. Protein-protein interaction analyses revealed the 2-isopropylmalate synthase (IPMS) protein as a common interactor partner of MtF-box and AtF-box, being a key enzyme in the biosynthesis pathway of the branched-chain amino acid leucine. For further detailed analysis, we focused on AtF-box and its role during the cell division cycle. Based on this work, we suggest a mechanism for the role of the studied F-box gene in regulation of leucine homeostasis, which is important for growth. © 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.
