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A common F-box gene regulates the leucine homeostasis of Medicago truncatula and Arabidopsis thaliana.

Authors
  • Iantcheva, Anelia1
  • Zhiponova, Miroslava2
  • Revalska, Miglena3
  • Heyman, Jefri4, 5
  • Dincheva, Ivayla3
  • Badjakov, Ilian3
  • De Geyter, Nathan4, 5
  • Boycheva, Irina6
  • Goormachtig, Sofie4, 5
  • De Veylder, Lieven4, 5
  • 1 AgroBioInstitute, Agricultural Academy, Blvd. Dragan Tsankov 8, 1164, Sofia, Bulgaria. [email protected] , (Bulgaria)
  • 2 Department of Plant Physiology, Faculty of Biology, Sofia University "St. Kliment Ohridski", 8 Dragan Tsankov blvd., 1164, Sofia, Bulgaria. , (Bulgaria)
  • 3 AgroBioInstitute, Agricultural Academy, Blvd. Dragan Tsankov 8, 1164, Sofia, Bulgaria. , (Bulgaria)
  • 4 Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052, Ghent, Belgium. , (Belgium)
  • 5 VIB Center for Plant Systems Biology, B-9052, Ghent, Belgium. , (Belgium)
  • 6 Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, Acad. Georgi Bonchev Str., Bl. 21, 1113, Sofia, Bulgaria. , (Bulgaria)
Type
Published Article
Journal
PROTOPLASMA
Publisher
Springer-Verlag
Publication Date
Mar 01, 2022
Volume
259
Issue
2
Pages
277–290
Identifiers
DOI: 10.1007/s00709-021-01662-w
PMID: 33973099
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

The F-box domain is a conserved structural protein motif that most frequently interacts with the SKP1 protein, the core of the SCFs (SKP1-CULLIN-F-box protein ligase) E3 ubiquitin protein ligases. As part of the SCF complexes, the various F-box proteins recruit substrates for degradation through ubiquitination. In this study, we functionally characterized an F-box gene (MtF-box) identified earlier in a population of Tnt1 retrotransposon-tagged mutants of Medicago truncatula and its Arabidopsis thaliana homolog (AtF-box) using gain- and loss-of-function plants. We highlighted the importance of MtF-box in leaf development of M. truncatula. Protein-protein interaction analyses revealed the 2-isopropylmalate synthase (IPMS) protein as a common interactor partner of MtF-box and AtF-box, being a key enzyme in the biosynthesis pathway of the branched-chain amino acid leucine. For further detailed analysis, we focused on AtF-box and its role during the cell division cycle. Based on this work, we suggest a mechanism for the role of the studied F-box gene in regulation of leucine homeostasis, which is important for growth. © 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.

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