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[Commitment to erythroid differentiation and globin gene expression in the absence of heme accumulation in Friend leukemia cells].

Authors
  • Gambari, R
Type
Published Article
Journal
Bollettino della Società italiana di biologia sperimentale
Publication Date
May 30, 1983
Volume
59
Issue
5
Pages
631–637
Identifiers
PMID: 6882562
Source
Medline
License
Unknown

Abstract

Friend leukemia cells (FLC) induced to erythroid differentiation by 5 mM hexamethylenebisacetamide (HMBA) are suitable for studies focused on the expression of different genes involved in the same differentiative program. In our studies FL cells have been cultured in alpha-medium (Gibco), 10% fetal calf serum (FCS, Flow Laboratories). Erythroid differentiation has been analysed by benzidine staining of the cells and globin synthesis by pulse-labelling the cells with 3H-leucine (New England Nuclear) and electrophoresis of newly-synthesized proteins on polyacrylamide gels. Hemoglobin production was determined by cellulose acetate gel electrophoresis. Our results demonstrate that heme synthesis is suppressed by the iron-chelator desferrioxamine. In addition "commitment" to erythroid differentiation and transcription and translation of globin mRNAs occur in HMBA-treated FLC in the absence of heme accumulation.

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