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Commercial formulation amendment transiently affects the microbial composition but not the biogas production of a full scale methanogenic UASB reactor.

Authors
  • Cabezas, A1
  • Bovio, P2
  • Etchebehere, C2
  • 1 Environmental Microbiology and Biotechnology Laboratory, Department of Environmental Sciences, Uruguay Technological University (UTEC), Durazno, Uruguay. , (Uruguay)
  • 2 Microbial Ecology Laboratory, Department of Biochemistry and Microbial Genetics, Biological Research Institute "Clemente Estable", Montevideo, Uruguay. , (Uruguay)
Type
Published Article
Journal
Environmental Technology
Publisher
Informa UK (Taylor & Francis)
Publication Date
Oct 01, 2020
Volume
41
Issue
24
Pages
3119–3133
Identifiers
DOI: 10.1080/09593330.2019.1600042
PMID: 30919752
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

The treatment of dairy wastewater in methanogenic reactors cause several problems due to their high lipid content. One strategy to overcome these problems is the use of commercial formulations. Here we studied the effect of adding a commercial formulation, designed to improve fat degradation, on both the microbial community composition and reactor performance. Samples from two full-scale Up-flow Anaerobic Sludge Blanket (UASB) reactors in parallel arrangement were analysed. The commercial product was added to one of the reactors while the other was used as control. The amendment increased significantly the fat removal but an accumulation of volatile fatty acids was detected. Nevertheless, no significant differences were observed in the total Chemical Oxygen Demand (COD) removal and biogas production between reactors. A significant change in the bacterial community was not detected by 16S rRNA gene Terminal Restriction Fragment Length Polymorphism (T-RFLP) analysis probably due to the limitation of the technique. A strong change in the composition of the phylum Firmicutes was detected with 16S rRNA gene amplicon sequencing; however, it didn't persist during the whole operation period. The relative abundance of minor Operational Taxonomic Units (OTUs) with sequences related to syntrophic bacteria increased with the amendment. Although a better hydrolytic capacity was obtained when adding the commercial product, the overall process did not improve and no increase in biogas production was detected. Alternative strategies could be applied to avoid the accumulation of intermediary products and improve biogas production as intermittent addition of the commercial product or batch operation of reactors.

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