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Comet assay reveals no genotoxicity risk of cationic solid lipid nanoparticles.

Authors
  • 1
  • 1 Institute of Biotechnology and Bioengineering, Centre of Genomics and Biotechnology, University of Trás-os-Montes and Alto Douro, 5001-801, Vila-Real, Portugal; Department of Biology and Environment, School of Life and Environmental Sciences, University of Trás-os-Montes and Alto Douro, 5001-801, Vila Real, Portugal. , (Portugal)
Type
Published Article
Journal
Journal of Applied Toxicology
1099-1263
Publisher
Wiley Blackwell (John Wiley & Sons)
Publication Date
Volume
34
Issue
4
Pages
395–403
Identifiers
DOI: 10.1002/jat.2961
PMID: 24243595
Source
Medline
Keywords
License
Unknown

Abstract

Cationic solid lipid nanoparticles (cSLN) are colloidal carriers for genes or drugs, particularly lipophilic drugs. Several reports exist on their high efficiency, but only a few studies report the effect of cSLNs on living cells. In the present work, internalization, cell viability (alamar blue assay) and genotoxic potential (alkaline comet assay) of three cSLN formulations (A-C) were evaluated in HepG2 and Caco-2 cells. cSLN showed an average hydrodynamic diameter (z-ave) of 141-222 nm, zeta-potential of 55.0-72.5 mV and polidispersity indices (PdI) of 0.336-0.421. Dispersion in physiological buffers increased z-ave and PdI. 0.01 mg ml(-1) cSLN unaffected cell viability, but 1.0 mg ml(-1) significantly decreased it, being cSLN-C (Compritol-based) the most toxic and HepG2 the most affected. DNA damage was not significantly increased by 0.1 mg ml(-1) cSLN but damage was observed at 1.0 mg ml(-1) cSLN-C. Thus, no genotoxicity is to be expected at concentrations that do not reduce cell viability.

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