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Comet assay of fresh and cryopreserved bull spermatozoa.

Authors
  • Słowińska, Mariola
  • Karol, Halina
  • Ciereszko, Andrzej
Type
Published Article
Journal
Cryobiology
Publisher
Elsevier
Publication Date
Feb 01, 2008
Volume
56
Issue
1
Pages
100–102
Identifiers
PMID: 18155688
Source
Medline
License
Unknown

Abstract

In this study, we describe DNA fragmentation of fresh and cryopreserved bull spermatozoa using the comet assay. Cryopreservation caused a significant but low (3.8%) decrease in the percentage of DNA in the comet head and an increase (5.3%) in the tail length. Our results suggest that in addition to motility and viability, low levels of DNA fragmentation after cryopreservation is a characteristic of bull spermatozoa and can be a part of remarkable cryoresistance of bull spermatozoa.

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