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Combining ultrasound and lactobacilli treatment for high-fat-diet-induced obesity in mice.

  • Liao, A-H1, 2
  • Jiang, C-B3, 4
  • Li, C-C1, 5
  • Chuang, H-C5
  • Chiang Chiau, J-S6
  • Chan, W-T3, 4
  • Yeung, C-Y3, 7
  • Cheng, M-L6
  • Lee, H-C7, 8, 9
  • 1 Graduate Institute of Biomedical Engineering, National Taiwan University of Science and Technology, Taipei, Taiwan. , (Taiwan)
  • 2 Department of medical engineering, National Defense Medical Center, Taipei, Taiwan. , (Taiwan)
  • 3 Department of Pediatrics, Taipei MacKay Memorial Hospital, Taipei, Taiwan. , (Taiwan)
  • 4 MacKay Junior College of Medicine, Nursing and Management, New Taipei City, Taiwan. , (Taiwan)
  • 5 Graduate Institute of Manufacturing Technology, National Taipei University of Technology, Taipei, Taiwan. , (Taiwan)
  • 6 Department of Medical Research, Hsinchu MacKay Memorial Hospital, Hsinchu, Taiwan. , (Taiwan)
  • 7 Department of Pediatrics, Taipei Medical School, Taipei City, Taiwan. , (Taiwan)
  • 8 Department of Pediatrics, MacKay Medical College, New Taipei City, Taiwan. , (Taiwan)
  • 9 Division of Gastroenterology and Nutrition, Department of Pediatrics, Hsinchu MacKay Memorial Hospital, Hsinchu, Taiwan. , (Taiwan)
Published Article
Journal of animal physiology and animal nutrition
Publication Date
Aug 01, 2017
DOI: 10.1111/jpn.12499
PMID: 27020164


Chronic systemic lipopolysaccharide-induced inflammation can cause obesity. In animal experiments, lactobacilli have been shown to inhibit obesity by modifying the gut microbiota, controlling inflammation and influencing the associated gene expression. A previous study found that high-fat-diet-induced (HFD) obesity was suppressed by lactobacilli ingestion in rats via the inhibition of parasympathetic nerve activity. This study explored the combined use of lactobacilli ingestion and ultrasound (US) to control body weight and body fat deposition in HFD mice over an 8-week experimental period. Male C57BL/6J mice received an HFD during treatment and were randomly divided into four groups: (i) control group (H), (ii) lactobacilli alone (HB), (iii) US alone (HU) and (iv) lactobacilli combined with US (HUB). The US was targeted at the inguinal portion of the epididymal fat pad on the right side. At the 8th week, body weight had decreased significantly in the HUB group (15.56 ± 1.18%, mean ± SD) group compared with the HU (26.63 ± 0.96%) and H (32.62 ± 5.03%) groups (p < 0.05). High-resolution microcomputed tomography (micro-CT) scans revealed that the reduction in total body fat volume was significantly greater in the HUB group (69%) than in the other two experimental groups (HB, 52%; HU, 37%; p < 0.05). The reductions in the thickness of the subcutaneous epididymal fat pads were significantly greater in the HUB group (final thickness: 340 ± 7 μm) than in the H (final thickness: 1150 ± 21 μm), HB (final thickness: 1060 ± 18 μm) and HU (final thickness: 370 ± 5 μm) groups (all p < 0.05). Combination therapy with lactobacilli and US appears to enhance the reduction in body weight, total and local body fat deposition, adipocyte size and plasma lipid levels over an 8-week period over that achieved with lactobacilli or US alone in HFD mice. These results indicate that US treatment alone can reduce hyperlipidemia in HFD mice.

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