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Combined effects of green tea supplementation and eccentric exercise on nuclear factor erythroid 2-related factor 2 activity.

Authors
  • Thorley, Josh1
  • Thomas, Craig1
  • Thon, Nicolas1
  • Nuttall, Hannah1
  • Martin, Neil R W1
  • Bishop, Nicolette1
  • Bailey, Stephen J1
  • Clifford, Tom2
  • 1 School of Sport, Exercise and Health Sciences, Loughborough University, Loughborough, LE11 3TU, UK.
  • 2 School of Sport, Exercise and Health Sciences, Loughborough University, Loughborough, LE11 3TU, UK. [email protected].
Type
Published Article
Journal
European Journal of Applied Physiology and Occupational Physiology
Publisher
Springer-Verlag
Publication Date
Jan 01, 2024
Volume
124
Issue
1
Pages
245–256
Identifiers
DOI: 10.1007/s00421-023-05271-8
PMID: 37439906
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

This study investigated whether combining eccentric exercise and green tea supplementation synergistically increased nuclear factor erythroid 2-related factor 2 (NRF2) activity, a transcription factor responsible for coordinating endogenous antioxidant expression. In a double-blinded, randomized, between-subjects design, 24 males (mean [SD]; 23 [3] years, 179.6 [6.1] cm, 78.8 [10.6] kg) performed 100 drop jumps following a 6 days supplementation period with either green tea (poly)phenols (n = 12; 500 mg·d-1) or a placebo (n = 12; inulin). NRF2/antioxidant response element (ARE) binding in peripheral blood mononuclear cells (PBMCs), catalase (CAT) and glutathione reductase (GR) activity, 8-hydroxy-2'-deoxyguanosine (8-OHdG) excretion, and differential leukocyte counts were measured pre-, post-, 1 h and 24 h post-exercise. Exercise did not increase NRF2/ARE binding (p = 0.12) (fold change vs rest: green tea = [post] 0.78 ± 0.45, [1 h] 1.17 ± 0.54, [24 h] 1.06 ± 0.56; placebo = [post] 1.40 ± 1.50, [1 h] 2.98 ± 3.70, [24 h] 1.04 ± 0.45). Furthermore, CAT activity (p = 0.12) and 8-OHdG excretion (p = 0.42) were unchanged in response to exercise and were not augmented by green tea supplementation (p > 0.05 for all). Exercise increased GR activity by 30% (p = 0.01), however no differences were found between supplement groups (p = 0.51). Leukocyte and neutrophil concentrations were only elevated post-exercise (p < 0.001 for all). Eccentric exercise, either performed alone or in conjunction with green tea supplementation, did not significantly increase NRF2 activity in PBMCs. osf.io/kz37g (registered: 15/09/21). © 2023. The Author(s).

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