Affordable Access

deepdyve-link
Publisher Website

Combination of Cinobufacini and Doxorubicin Increases Apoptosis of Hepatocellular Carcinoma Cells through the Fas- and Mitochondria-Mediated Pathways.

Authors
  • Xia, Jufeng1
  • Inagaki, Yoshinori1
  • Gao, Jianjun1, 2
  • Qi, Fanghua1, 3
  • Song, Peipei1
  • Han, Guohua1
  • Sawakami, Tatsuo1
  • Gao, Bo4
  • Luo, Chuan4
  • Kokudo, Norihiro1
  • Hasegawa, Kiyoshi1
  • Sakamoto, Yoshihiro1
  • Tang, Wei1
  • 1 * Hepato-Biliary-Pancreatic Surgery Division, Department of Surgery, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan. , (Japan)
  • 2 † Department of Pharmacology, School of Pharmaceutical Sciences, Qingdao University, Qingdao, Shandong, China. , (China)
  • 3 ‡ Department of Traditional Chinese Medicine, Shandong Provincial Hospital Affiliated to Shandong University, Ji'nan, China. , (China)
  • 4 § Anhui Jinchan Biochemistry Company, Huaibei, Anhui, China. , (China)
Type
Published Article
Journal
The American journal of Chinese medicine
Publication Date
Jan 01, 2017
Volume
45
Issue
7
Pages
1537–1556
Identifiers
DOI: 10.1142/S0192415X17500835
PMID: 28946772
Source
Medline
Keywords
License
Unknown

Abstract

Cinobufacini, a traditional Chinese medicine, has been used widely for cancer treatment, such as hepatocellular carcinoma (HCC), sarcoma, and leukemia. Previous studies done by our lab indicated that cinobufacini could suppress HCC cells through mitochondria-mediated and Fas-mediated apoptotic pathways. Here, we use a combination of cinobufacini and doxorubicin to inhibit the growth of HCC cells. The combination group induced more significant apoptosis by affecting proteins and RNA of apoptosis-related elements, such as Bcl-2, Bax, Bid, and cytochrome c. Furthermore, cinobufacini, as a mixture of a number of components, had stronger apoptosis-inducing activity than particular individual components or a simple mixture of a few components. Overall, these results suggested that the combination of cinobufacini and doxorubicin may provide a new strategy for inhibiting the proliferation of HCC cells.

Report this publication

Statistics

Seen <100 times