The use of colors in the environment of an Intensive Care Unit (ICU) may interfere in the welfare of both professionals and patients. This study is aimed at analyzing the perceptions of professionals and patients regarding the colors used in the intensive care environment, identifying those considered pleasant and unpleasant. This is a descriptive-exploratory research with a quantitative-qualitative focus. The sample was comprised of hospitalized patients and professionals at three public hospitals ICUs in the city of Goiânia, State of Goiás. The colors considered most pleasant for ICUs were light blue and light green. Light yellow, beige, gray, pink and guava were also pointed out as pleasing. Red and black were appointed as the most unpleasant. Professionals and patients demonstrated preference for varied colors, something that can be used as a way of improving the atmosphere of ICUs.