Affordable Access

Access to the full text

Colorimetric and fluorescent dual-identification of glutathione based on its inhibition on the 3D ball-flower shaped Cu-hemin-MOF’s peroxidase-like activity

Authors
  • Chen, Xiaolong1
  • Wang, Xianfeng1
  • Cao, Gaihua1
  • Wu, Yawen1
  • Luo, Huibo2
  • Ji, Zhong1
  • Shen, Caihong1, 3
  • Huo, Danqun1, 2
  • Hou, Changjun1, 1
  • 1 Chongqing University, Chongqing, 400044, People’s Republic of China , Chongqing (China)
  • 2 Sichuan University of Science and Engineering, Zigong, 643000, People’s Republic of China , Zigong (China)
  • 3 Luzhou Laojiao Group Co. Ltd., Luzhou, 646000, People’s Republic of China , Luzhou (China)
Type
Published Article
Journal
Microchimica Acta
Publisher
Springer-Verlag
Publication Date
Oct 09, 2020
Volume
187
Issue
11
Identifiers
DOI: 10.1007/s00604-020-04565-4
Source
Springer Nature
Keywords
License
Yellow

Abstract

Hemin as the organic linker ligand and Cu (II) as the metal center were applied to prepare a copper-metal-organic framework (Cu-hemin-MOF) via one-step hydrothermal method. Characterization using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), and X-ray powder diffraction (XRD) demonstrate that the acquired Cu-hemin-MOF possesses the appearance of 3D ball-flower shape with the existence of C, N, O, Fe, and Cu on the surface. Further study found that this 3D ball-flower shaped Cu-hemin-MOF exhibits peroxidase-like activity, which can catalyze the peroxidase substrate of o-phenylenediamine (OPD) to generate 2,3-diaminophenazine (DAP) in the presence of H2O2. DAP has a yellow color and also emits a strong fluorescence when excited by ultraviolet light. Interestingly, Cu-hemin-MOF’s peroxidase-like activity can be strongly inhibited by glutathione (GSH). In view of this, a dual readout (fluorescence and colorimetry) was proposed to detect GSH for the first time. Under optimal conditions, the proposed method exhibits good linear relationship between the signal response (fluorescence and colorimetry) and the concentration of GSH, and low limits of detection (LOD) of 2.3 and 26.6 nM, respectively. Graphical abstract

Report this publication

Statistics

Seen <100 times