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A colorimetric aptamer biosensor based on cationic polythiophene derivative as peroxidase mimetics for the ultrasensitive detection of thrombin.

Authors
  • Liu, Mei1
  • Li, Jiao2
  • Li, Baoxin3
  • 1 Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi'an 710062, China. Electronic address: [email protected] , (China)
  • 2 Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi'an 710062, China. , (China)
  • 3 Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry&Chemical Engineering, Shaanxi Normal University, Xi'an 710062, China. Electronic address: [email protected] , (China)
Type
Published Article
Journal
Talanta
Publication Date
Dec 01, 2017
Volume
175
Pages
224–228
Identifiers
DOI: 10.1016/j.talanta.2017.07.003
PMID: 28841983
Source
Medline
Keywords
License
Unknown

Abstract

A colorimetric assay for the ultrasensitive determination of thrombin was presented, in which the cationic polythiophene derivative was used as catalyst of the 3,3',5,5'-tetramethylbenzidine (TMB)-H2O2 reaction and the thrombin-binding aptamer (TBA) was used as inducing polymer's different conformation elements. It was found the cationic polythiophene derivative, poly[3-(3'-N,N,N-triethylamino-1'-propyloxy)-4-methyl-2,5-t-hiophene hydrochloride] (PMNT), can catalyze the oxidation reaction of TMB in the presence of H2O2 to produce a blue color solution. The catalytic activity of PMNT on the TMB-H2O2 reaction was closely relevant to the conformation of PMNT. The absorbance of TMB-H2O2 was distinctly increased in the presence of TBA. With the addition of thrombin, TBA interacted with thrombin to form a G-quadruplex structure. The conformational change weakened the catalytic activity of PMNT and resulted in a decrease in the absorbance. The colorimetric sensor could detect thrombin down to 4pM with high selectivity against other interfering proteins. This work is not only of importance for a better understanding of the unique properties of cationic polythiophenes derivative but also have great potential for medical diagnostics and therapy for human health.

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