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Coimmunoprecipitation and proteomic analyses.

Authors
  • Falsone, S Fabio
  • Gesslbauer, Bernd
  • Kungl, Andreas J
Type
Published Article
Journal
Methods in Molecular Biology
Publication Date
Jan 01, 2008
Volume
439
Pages
291–308
Identifiers
DOI: 10.1007/978-1-59745-188-8_20
PMID: 18370111
Source
Medline
License
Unknown

Abstract

Defining protein-protein interaction networks is a major goal of proteomics. Here, we present a protocol for coimmunoprecipitation, a technique suitable for the isolation of whole protein complexes in vivo and their subsequent identification by either immunoblotting or mass spectrometric sequencing combined to database search.

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