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Coimmobilization of urease and glutamate dehydrogenase in electrochemically prepared polypyrrole-polyvinyl sulfonate films.

Authors
  • Gambhir, A
  • Gerard, M
  • Mulchandani, A K
  • Malhotra, B D
Type
Published Article
Journal
Applied biochemistry and biotechnology
Publication Date
Jan 01, 2001
Volume
96
Issue
1-3
Pages
249–257
Identifiers
PMID: 11783891
Source
Medline
License
Unknown

Abstract

Immobilization of urease and glutamate dehydrogenase enzymes in electrochemically prepared polypyrrole-polyvinyl sulfonate films (PPY-PVS) was carried out using physical adsorption and electrochemical entrapment techniques. Detailed studies on optimum pH, Fourier transform infrared spectroscopy, cyclic voltammetry, and scanning electron microscopy of the enzymes in the immobilized state were conducted. The value of the apparent Michaelis-Menten constant was experimentally determined to be 2.5 and 2.7 for physically adsorbed and electrochemically entrapped urease in PPY-PVS films, respectively.

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